Glasgow/Wetlab/Gels

(Difference between revisions)

Revision as of 14:46, 28 August 2007

Gels are labelled with numbers for rows and letters for columns.

Date and Reference	Description	Key
10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1.	1.(m) 2.(m) 3.(s) 4.(s) 5.(s) 6.(s) 7.neg control 8.neg control 9.neg control
10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2.	1.(s) 2.(s) 3.neg control 4.DntR 5.DntR 6.Pu 7.Pu 8.neg control 9.neg control 10.neg control 11.neg control
13th Aug 2007 (4)	PCR of genes (a→g), (a→d), and (d→g).	1A:(a→g) 1B:(a→g) 1C:(a→g) 2A:(a→g) 2B:(a→d) 2C:(d→g)
17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1.	1.-3.(m)+4/6B (PstI site mutated) 4.-6.(m)+3/20G (PstI site mutated) 7.-9.(s)+4/6B (1st PstI site mutated) 10.-12.(s)+3/20G (2nd PstI site mutated) 13.-15.(s)+4/6B (2nd PstI site mutated) 16.(s)+3/20G (1st PstI site mutated)
17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2.	1.-2.(s)+3/20G (1st PstI site mutated) 3.(m)+4/6B (Before mutagenesis) 4.(m)+3/20G (Before mutagenesis) 5.(s)+4/6B (Before mutagenesis) 6.(s)+3/20G (Before mutagenesis)

@@ Line 11: / Line 11: @@
 |[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]]
 |Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1.
-|1.(*m*)<br>
+|'''1.'''(*m*)<br>
-.(*m*)<br>
+'''2.'''(*m*)<br>
-.(*s*)<br>
+'''3.'''(*s*)<br>
-.(*s*)<br>
+'''4.'''(*s*)<br>
-.(*s*)<br>
+'''5.'''(*s*)<br>
-.(*s*)<br>
+'''6.'''(*s*)<br>
-.neg control<br>
+'''7.'''neg control<br>
-.neg control<br>
+'''8.'''neg control<br>
-.neg control<br>
+'''9.'''neg control<br>
 |[[Image:10thAug gel 1.JPG|200px]]
 |-

Date and Reference	Description	Key
10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1.	1.(m) 2.(m) 3.(s) 4.(s) 5.(s) 6.(s) 7.neg control 8.neg control 9.neg control
10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2.	1.(s) 2.(s) 3.neg control 4.DntR 5.DntR 6.Pu 7.Pu 8.neg control 9.neg control 10.neg control 11.neg control
13th Aug 2007 (4)	PCR of genes (a→g), (a→d), and (d→g).	1A:(a→g) 1B:(a→g) 1C:(a→g) 2A:(a→g) 2B:(a→d) 2C:(d→g)
17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1.	1.-3.(m)+4/6B (PstI site mutated) 4.-6.(m)+3/20G (PstI site mutated) 7.-9.(s)+4/6B (1st PstI site mutated) 10.-12.(s)+3/20G (2nd PstI site mutated) 13.-15.(s)+4/6B (2nd PstI site mutated) 16.(s)+3/20G (1st PstI site mutated)
17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2.	1.-2.(s)+3/20G (1st PstI site mutated) 3.(m)+4/6B (Before mutagenesis) 4.(m)+3/20G (Before mutagenesis) 5.(s)+4/6B (Before mutagenesis) 6.(s)+3/20G (Before mutagenesis)