Revision as of 14:09, 29 August 2007

Gels

Date and Reference	Description	Key	Gel
10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1.	1.(m) 2.(m) 3.(s) 4.(s) 5.(s) 6.(s) 7.neg control 8.neg control 9.neg control
10th Aug 2007 (2)	Colony PCR after ligating (m), (s), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2.	1.(s) 2.(s) 3.neg control 4.DntR 5.DntR 6.Pu 7.Pu 8.neg control 9.neg control 10.neg control 11.neg control

Date and Reference	Description	Key	Gel
13th Aug 2007 (4)	PCR of genes (a→g), (a→d), and (d→g).	1A.(a→g) 1B.(a→g) 1C.(a→g) 2A.(a→g) 2B.(a→d) 2C.(d→g)

Date and Reference	Description	Key	Gel
17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1.	1.-3.(m)+4/6B (PstI site mutated) 4.-6.(m)+3/20G (PstI site mutated) 7.-9.(s)+4/6B (1st PstI site mutated) 10.-12.(s)+3/20G (2nd PstI site mutated) 13.-15.(s)+4/6B (2nd PstI site mutated) 16.(s)+3/20G (1st PstI site mutated)
17th Aug 2007 (2-4)	PstI digested minipreps of (m) and (s) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2.	1.-2.(s)+3/20G (1st PstI site mutated) 3.(m)+4/6B (Before mutagenesis) 4.(m)+3/20G (Before mutagenesis) 5.(s)+4/6B (Before mutagenesis) 6.(s)+3/20G (Before mutagenesis)

Date and Reference	Description	Key
23rd Aug 2007	(s) minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis.	1.-5.(s)+4/6B 6.-16.(s)+3/20G
23rd Aug 2007	PstI digested (s) minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis.	1.-5.(s)+4/6B 6.-16.(s)+3/20G
23rd Aug 2007	Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 1.	1.-7.colonies from the plate
23rd Aug 2007	Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 2.	8.-12.colonies from the plate 13.neg control

Date and Reference	Description	Key	Gel
24th Aug 2007 (1)	These are the miniprepped plasmids from Pr+XylR in TOPO vector. The seven colonies gave the right size product from colony PCR but failed to give right size bands after miniprepping. Wait for sequencing results.	1.-7.colonies from Pr+XylR in TOPO transformations

Date and Reference	Description	Key	Gel
27th Aug 2007 (1)	pDntA in 3/20G and 4/6B vectors digested with Roche's PvuI restriction enzyme. Results do not match up with expected fragment sizes, thus performing a PCR with "VF2" and "VR" primers.	1.pDntA+3/20G 2.pDntA+4/6B

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 '''13.-15.'''(*s*)+4/6B<br>(2nd PstI site mutated)<br>
 '''16.'''(*s*)+3/20G<br>(1st PstI site mutated)<br>
-|[[Image:17thAug SDM1 PstI digest gel 1.JPG|200px]]
+|[[Image:17thAug SDM1 PstI digest gel 1.JPG|220px]]
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 |[[Glasgow/Wetlab/Week7#Friday 17th August 2007|17th Aug 2007 (2-4)]]