Glasgow/Wetlab/Week9
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| - | [[User:Christinemerrick|Christine] digested minipreps of interest B, C and D suspected to contain (*d→g*) from 24/08/07 with PstI. Miniprep of colony D shows bands of expeceted sizes 1155bp, 549bp, ~1962bp, and 3682bp. | + | [[User:Christinemerrick|Christine]] digested minipreps of interest B, C and D suspected to contain (*d→g*) from 24/08/07 with PstI. Miniprep of colony D shows bands of expeceted sizes 1155bp, 549bp, ~1962bp, and 3682bp. |
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| + | Overnights were also set up of colonies K, L, M, N, Q, S and T, each suspected of containing (*a→d*), from plate 2. | ||
=== Tuesday 28 August 2007 === | === Tuesday 28 August 2007 === | ||
Revision as of 17:20, 30 August 2007
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Week 9
Monday 27th August 2007
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Maija performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme. The bands were weird sizes, see the gel here.
Insert Vector Enzyme Expected size Result pDntA 3/20G PvuI 1450bp + 749bp ~2500bp pDntA 4/6B PvuI 2375bp + 681bp ~3800bp + ~1250bp - Christine digested minipreps of interest B, C and D suspected to contain (*d→g*) from 24/08/07 with PstI. Miniprep of colony D shows bands of expeceted sizes 1155bp, 549bp, ~1962bp, and 3682bp.
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Overnights were also set up of colonies K, L, M, N, Q, S and T, each suspected of containing (*a→d*), from plate 2.
Tuesday 28 August 2007
Wednesday 29th August 2007
Scott did colony PCR (see protocol 9) on the assembly transformations carried out yesterday using primers VF2 and VR. Unfortunately it looks as though the combined product is larger than the sum of its parts...
Scott also did the following restriction digests (see Protocol 7):
Insert Digest 1 (EcoRI/PvuI) Insert Digest 2 (EcoRI/SpeI)
(Done overnight at 16°C)J23119 (tube 1) J61101 J23119 (tube 2) "
Destination (EcoRI/XbaI)
(Done overnight at 16°C)J61101
Thursday 30th August 2007
Friday 31st August 2007
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