Glasgow/Wetlab/Gels
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|[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]] | |[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]] | ||
- | |Colony PCR after ligating | + | |Colony PCR after ligating phzM, phzS, DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. |
- | |'''1.''' | + | |'''1.'''phzM<br> |
- | '''2.''' | + | '''2.'''phzM<br> |
- | '''3.''' | + | '''3.'''phzS<br> |
- | '''4.''' | + | '''4.'''phzS<br> |
- | '''5.''' | + | '''5.'''phzS<br> |
- | '''6.''' | + | '''6.'''phzS<br> |
'''7.'''neg control<br> | '''7.'''neg control<br> | ||
'''8.'''neg control<br> | '''8.'''neg control<br> | ||
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|[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]] | |[[Glasgow/Wetlab/Week6#Friday 10th August 2007|10th Aug 2007 (2)]] | ||
- | |Colony PCR after ligating | + | |Colony PCR after ligating phzM, phzS, DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. |
- | |'''1.''' | + | |'''1.'''phzS<br> |
- | '''2.''' | + | '''2.'''phzS<br> |
'''3.'''neg control<br> | '''3.'''neg control<br> | ||
'''4.'''DntR<br> | '''4.'''DntR<br> |
Revision as of 14:45, 21 October 2007
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Contents[hide] |
Gels
Friday 10th August 2007
Date and Reference | Description | Key | Gel |
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10th Aug 2007 (2) | Colony PCR after ligating phzM, phzS, DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. | 1.phzM 2.phzM | |
10th Aug 2007 (2) | Colony PCR after ligating phzM, phzS, DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. | 1.phzS 2.phzS |
Monday 13th August 2007
Date and Reference | Description | Key | Gel |
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13th Aug 2007 (4) | PCR of genes (*a→g*), (*a→d*), and (*d→g*). Wells in the gel were made larger by putting tape on the combs in order to yield more DNA in gel extraction. | 1A.(*a→g*) 1B.(*a→g*) |
Friday 17th August 2007
Date and Reference | Description | Key | Gel |
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17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. | 1.-3.(*m*)+4/6B (PstI site mutated) 4.-6.(*m*)+3/20G | |
17th Aug 2007 (2-4) | PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. | 1.-2.(*s*)+3/20G (1st PstI site mutated) 3.(*m*)+4/6B |
Thursday 23rd August 2007
Date and Reference | Description | Key | Gel |
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23rd Aug 2007 | (*s*) minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis. | 1.-5.(*s*)+4/6B 6.-16.(*s*)+3/20G | |
23rd Aug 2007 | PstI digested (*s*) minipreps in 4/6B and 3/20G vectors after the second round of site-directed mutagenesis. | 1.-5.(*s*)+4/6B 6.-16.(*s*)+3/20G | |
23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 1. | 1.-7.colonies from the plate | |
23rd Aug 2007 | Colony PCR with "Pr_Prefix" and "XylR_Suffix" primers from Lynsey's transformations with Pr+XylR miniprep DNA. Gel 2. | 8.-12.colonies from the plate 13.neg control |
Friday 24th August 2007
Date and Reference | Description | Key | Gel |
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24th Aug 2007 (1) | These are the miniprepped plasmids from Pr+XylR in TOPO vector. The seven colonies gave the right size product from colony PCR but failed to give right size bands after miniprepping. Wait for sequencing results. | 1.-7.colonies from Pr+XylR in TOPO transformations |
Monday 27th August 2007
Date and Reference | Description | Key | Gel |
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27th Aug 2007 (1) | pDntA in 3/20G and 4/6B vectors digested with Roche's PvuI restriction enzyme. Results do not match up with expected fragment sizes, thus performing a PCR with "VF2" and "VR" primers. | 1.pDntA+3/20G 2.pDntA+4/6B |
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