E-Notebook
From 2007.igem.org
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== Tasks Accomplished == | == Tasks Accomplished == | ||
| + | === Experiments so far === | ||
| + | #Calibration of the number of colonies obtained at different optical densities. | ||
| + | #Transformation of competent E.coli (strain K12Z1) cells with constructs. | ||
| + | #Induction of the construct pLac cfp | ||
| + | E.coli cells transformed with the construct pLac cfp were cultured for 10 hours in Luria Bertani medium containing 50mg/ml Ampicillin. | ||
| + | The cells were then transferred into a minimal medium (M9 medium) and were induced with the following concentrations of IPTG. A stock concentration of 100mM was used | ||
| + | {| cellpadding="2" cellspacing="3" border="1" | ||
| + | !Sample | ||
| + | !100mM IPTG (ul) | ||
| + | !1mM IPTG (ul) | ||
| + | !Inoculum volume from LB (ul) | ||
| + | !Volume of M9 added (ml) | ||
| + | |-align="center" | ||
| + | |pLac cfp (0 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |-align="center" | ||
| + | |pLac cfp (1 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |-align="center" | ||
| + | |pLac cfp (10 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |-align="center" | ||
| + | |pLac cfp (50 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |-align="center" | ||
| + | |pLac cfp (100 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |-align="center" | ||
| + | |pLac cfp (1000 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |-align="center" | ||
| + | |pLac cfp (0 uM) | ||
| + | | - | ||
| + | | - | ||
| + | |3 ul | ||
| + | |3 ml | ||
| + | |} | ||
== The Week Ahead == | == The Week Ahead == | ||
Revision as of 06:57, 27 June 2007
| The official wiki of the NCBS iGEM 2007 Team |
| [http://www.ncbs.res.in/ National Centre for Biological Sciences, Bangalore] |
|---|
| Bangalore | The Company | The Mission | e-Notebook |
|---|
| The Bangalore iGEM Journal, 07
|
Contents |
e-notebook
| May 07 | June 07 | July 07 | ||||||||||||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| S | M | T | W | T | F | S | S | M | T | W | T | F | S | S | M | T | W | T | F | S | ||||||||||||||
| 1 | 2 | 3 | 4 | 5 | 1 | 2 | 1 | 2 | 3 | 4 | 5 | 6 | 7 | |||||||||||||||||||||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | ||||||||||||||
| 13 | 14 | 15 | 16 | 17 | 18 | 19 | 10 | 11 | 12 | 13 | 14 | 15 | 16 | 15 | 16 | 17 | 18 | 19 | 20 | 21 | ||||||||||||||
| 20 | 21 | 22 | 23 | 24 | 25 | 26 | 17 | 18 | 19 | 20 | 21 | 22 | 23 | 22 | 23 | 24 | 25 | 26 | 27 | 28 | ||||||||||||||
| 27 | 28 | 29 | 30 | 31 | 24 | 25 | 26 | 27 | 28 | 29 | 30 | 29 | 30 | |||||||||||||||||||||
Current Status
Tasks Accomplished
Experiments so far
- Calibration of the number of colonies obtained at different optical densities.
- Transformation of competent E.coli (strain K12Z1) cells with constructs.
- Induction of the construct pLac cfp
E.coli cells transformed with the construct pLac cfp were cultured for 10 hours in Luria Bertani medium containing 50mg/ml Ampicillin.
The cells were then transferred into a minimal medium (M9 medium) and were induced with the following concentrations of IPTG. A stock concentration of 100mM was used
| Sample | 100mM IPTG (ul) | 1mM IPTG (ul) | Inoculum volume from LB (ul) | Volume of M9 added (ml) |
|---|---|---|---|---|
| pLac cfp (0 uM) | - | - | 3 ul | 3 ml |
| pLac cfp (1 uM) | - | - | 3 ul | 3 ml |
| pLac cfp (10 uM) | - | - | 3 ul | 3 ml |
| pLac cfp (50 uM) | - | - | 3 ul | 3 ml |
| pLac cfp (100 uM) | - | - | 3 ul | 3 ml |
| pLac cfp (1000 uM) | - | - | 3 ul | 3 ml |
| pLac cfp (0 uM) | - | - | 3 ul | 3 ml |
