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Revision as of 18:39, 4 July 2007 (view source) Klapoetke (Talk | contribs) ← Older edit		Revision as of 21:59, 4 July 2007 (view source) Klapoetke (Talk | contribs) Newer edit →
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	===Endonuclease Digestion===		===Endonuclease Digestion===
-	*10uL H2O	+	*10uL H<sub>2</sub>O
	*5uL DNA		*5uL DNA
	*2uL 10x buffer		*2uL 10x buffer

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Revision as of 21:59, 4 July 2007

Endonuclease Digestion

• 10uL H₂O
• 5uL DNA
• 2uL 10x buffer
• 2uL 1:10 dilution of BSA soln
• 0.5uL Enzyme 1
• 0.5uL Enzyme 2

Incubate at 37C for 2-4 hours

Use [http://www.promega.com/guides/re_guide/RESearch.asp this website] for restriction enzyme and [http://www.promega.com/guides/re_guide/RESearch.asp?search=buffer this site] for checking buffer compatibility.