From 2007.igem.org

PROTOCOLSREFERENCES

Week 1

03/07

Maija and Christine prepared LB broth and LB agar with Protocol 1. Made 4 batches of plates, 2 with kanamycin and 2 with carbenicillin.

04/07

1. All wetlab researched BioBricks.
2. Reporter constructs and mini-Tn5 stocks looked out.
3. Streaked the following:
   1. P. putida PAW 340 pJAK14 Carb. plate
   2. Tn5 lux AB Carb. plate
   3. Mini-Tn5 lux AB Carb. plate
   4. P. Fluorescens NCIMB 9815 Carb. plate
   5. P. putida KT2440 LB
   6. JM109 pBluescript 5k+ Carb. plate
   7. Mini-Tn5 Tc Carb. Plate
   8. pQF52 Carb. plate
   9. P. Fluorescens 9815 LB
   10. E. coli pJAK14 Km plate
   11. Il DntR in pOF52 Carb. plate
   12. pUCINR in Ω strain C Carb. plate
   13. pGLTUR Carb. plate
   14. Mini-Tn5 Kan Carb. plate
   15. Mini-Tn5 Sm/Sp Carb. plate
   16. Mini-Tn5 1cc2 Carb. plate
   17. E. coli Sa1 LB
   18. DmpR #24 Carb. plate
   19. Mini-Tn5 lac 32 in E. coli 517 Carb. plate
   20. Mini-Tn5 Tc Carb. Plate
   21. Mini-Tn5 Cm Carb. plate
   22. DmpR WT Carb. plate
   23. E.coli sm 10 pESD15 Tn5 GFP Carb. plate
   24. pUJ8 Carb. Plate