From 2007.igem.org

Transforming BioBricks

1. Split the competent E. coli cells (~75ul) in two.
2. Competent cells (~33ul) into eppendorfs on ice.
3. Add 1µl of plasmid DNA.
4. Incubate on ice for at least 5 mins.
5. Heat shock +42 ºC for 45 secs.
6. Back on ice for 2 mins to reduce damages.
7. Add 1ml of soc media.
8. Incubate +37ºC in a shaker for 1 hour.
9. Spread the reaction on kan/carb LB plates.
10. Grow +37 ºC overnight.