| Name
| Sequence
| Tm
|
| *PhZ-UP
| 5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
| 65.6
|
| *PhZ-LOW
| 5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
| 64.8
|
| *BbpPhZ_Up
| 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
| 63.6
|
| *BbsPhZ_Low
| 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
| 64.2
|
| BbpGene
| 5' gaa ttc gcg gcc gct tct ag 3'
| 65.7
|
| Bbs
| 5' ctg cag cgg ccg cta cta gta 3'
| 65.3
|
| *METHYL1
| 5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
| 66.3
|
| *METHYL2
| 5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
| 66.2
|
| *BbpMethyl1
| 5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
| 63.9
|
| *BbsMethyl2
| 5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
| 66.9
|
| PhzMfor1
| 5' atg aat aat tcg aat ctt gct gct 3'
| 65.4
|
| PhzMrev1
| 5' tca ggc cct ggc agc gac 3'
| 60.3
|
| BbpPhzMfor1
| 5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3'
| 64.5
|
| BbsPhzMrev1
| 5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3'
| 60.3
|
| *OXY1
| 5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
| 60.2
|
| *OXY2
| 5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
| 59.2
|
| *BbpOxy1
| 5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
| 65.1
|
| *BbsOxy2
| 5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
| 66.9
|
| PhzSfor1
| 5' atg agc gaa ccc atc gat at 3'
| 71.2
|
| PhzSrev1
| 5' cta gcg tgg ccg ttc cac ctg 3'
| 60.2
|
| BbpPhzSfor1
| 5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3'
| 70.9
|
| BbsPhzSrev1
| 5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3'
| 63.3
|
| PhzH_up
| 5' CGC ACG GAT CCT TTC AGA ATG TTC 3'
| 69.9
|
| PhzH_low
| 5' GCC ACG CCA AGC TTC ACG CTC A 3'
| 70.6
|
| BbpPhZ_Up
| 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
| 63.3
|
| BbsPhZ-Low
| 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
| 57.7
|
