Glasgow/Wetlab/Orders
From 2007.igem.org
Emma's primers
| Name | Sequence | Tm |
|---|---|---|
| *PhZ-Up | 5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' | 65.6 |
| *PhZ-Low | 5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3' | 64.8 |
| *Bbp_PhZ_Up | 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' | 63.6 |
| *Bbs_PhZ_Low | 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' | 64.2 |
| Bbp_Gene | 5' gaa ttc gcg gcc gct tct ag 3' | 65.7 |
| Bbs | 5' ctg cag cgg ccg cta cta gta 3' | 65.3 |
| *Methyl_1 | 5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' | 66.3 |
| *Methyl_2 | 5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' | 66.2 |
| *Bbp_Methyl_1 | 5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3' | 63.9 |
| *Bbs_Methyl_2 | 5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3' | 66.9 |
| Phz_M_for_1 | 5' atg aat aat tcg aat ctt gct gct 3' | 65.4 |
| Phz_M_rev_1 | 5' tca ggc cct ggc agc gac 3' | 60.3 |
| Bbp_Phz_M_for_1 | 5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3' | 64.5 |
| Bbs_Phz_M_rev_1 | 5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3' | 60.3 |
| *Oxy_1 | 5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3' | 60.2 |
| *Oxy_2 | 5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' | 59.2 |
| *Bbp_Oxy_1 | 5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3' | 65.1 |
| *Bbs_Oxy_2 | 5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3' | 66.9 |
| Phz_S_for_1 | 5' atg agc gaa ccc atc gat at 3' | 71.2 |
| Phz_S_rev_1 | 5' cta gcg tgg ccg ttc cac ctg 3' | 60.2 |
| Bbp_Phz_S_for_1 | 5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3' | 70.9 |
| Bbs_Phz_S_rev_1 | 5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3' | 63.3 |
| Phz_H_up | 5' CGC ACG GAT CCT TTC AGA ATG TTC 3' | 69.9 |
| Phz_H_low | 5' GCC ACG CCA AGC TTC ACG CTC A 3' | 70.6 |
| Bbp_Phz_Up | 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3' | 63.3 |
| Bbs_Phz_Low | 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3' | 57.7 |
Primers marked with * are taken from previous studies.
