| Name
| Sequence
| Tm
|
| DmpR_SDM_PstI_1_for
| 5´CTGGAGCAACAGCATGCGCTGTTC3´
| 65.6
|
| DmpR_SDM_PstI_1_rev
| 5´GCTCCAGTTTTCAGCGATGGCC3´
| 64.8
|
| DmpR_SDM_PstI_2_for
| 5´CTGGAGGAAGTGCTCAACCAGCAG3´
| 66.3
|
| DmpR_SDM_PstI_2_rev
| 5´CCTCCAGCGCTTCCACCAG3´
| 66.2
|
| DmpR_Start_Prefix_1_for
| 5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´
| 60.2
|
| DmpR_End_Suffix_1_rev
| 5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´
| 59.2
|
| ***M_SDM_PstI_1_for
| 5´***3´
| 69.9
|
| ***M_SDM_PstI_1_rev
| 5´***3´
| 70.6
|
| ***S_SDM_PstI_1_for
| 5´***3´
| 65.7
|
| ***S_SDM_PstI_1_rev
| 5´***3´
| 65.3
|
| ***S_SDM_PstI_2_for
| 5´***3´
| 63.6
|
| ***S_SDM_PstI_2_rev
| 5´***3´
| 63.9
|
| ***C_SDM_PstI_1_for
| 5´***3´
| 65.1
|
| ***C_SDM_PstI_1_rev
| 5´***3´
| 64.2
|
| ***F_SDM_PstI_1_for
| 5´***3´
| 66.9
|
| ***F_SDM_PstI_1_rev
| 5´***3´
| 66.9
|
| ***B_SDM_EcoRI_1_for
| 5'***3'
| 65.4
|
| ***B_SDM_EcoRI_1_rev
| 5'***3'
| 64.5
|
| ***E_SDM_EcoRI_1_for
| 5'***3'
| 60.3
|
| ***E_SDM_EcoRI_1_rev
| 5'***3'
| 60.3
|
| ***E_SDM_PstI_1_for
| 5'***3'
| 71.2
|
| ***E_SDM_PstI_1_rev
| 5'***3'
| 70.9
|
| XylR_SDM_PstI_1_for
| 5´GAGCTCCAGACCCAGGTTG3´
| 63.3
|
| XylR_SDM_PstI_1_rev
| 5´GTCTGGAGCTCGTAGCGCTC3´
| 63.3
|
| XylR_Prefix_1
| 5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´
| 57.7
|
| XylR_Suffix_1
| 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´
| 59.8
|
| Pu_Prefix_1(AfterXylR)
| 5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´
| 65.0
|
| Pu_Suffix_1(AfterXylR)
| 5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´
| 65.1
|
| Pu_Prefix_2(Emma´s sequence)
| 5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´
| 66.0
|
| Pu_Suffix_2(Emma´s sequence)
| 5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´
| 66.4
|
