Glasgow/Wetlab/Week9
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Contents |
Week 9
Monday 27th August 2007
- Maija performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme. The bands were weird sizes, see the gel here.
| Insert | Vector | Enzyme | Expected size | Result |
|---|---|---|---|---|
| pDntA | 3/20G | PvuI | 1450bp + 749bp | ~2500bp |
| pDntA | 4/6B | PvuI | 2375bp + 681bp | ~3800bp + ~1250bp |
Tuesday 28 August 2007
Wednesday 29th August 2007
Scott did colony PCR (see protocol 9) on the assembly transformations carried out yesterday using primers VF2 and VR. Unfortunately it looks as though the combined product is larger than the sum of its parts...
Scott also did the following restriction digests (see Protocol 5):
| Insert Digest 1 (EcoRI/PvuI) | Insert Digest 2 (EcoRI/SpeI) |
| J23119 (tube 1) | J61101 |
| J23119 (tube 2) | " |
| Destination (EcoRI/XbaI) |
| J61101 |
"Insert Digest 2" and the "Destination Digest" were both done overnight at 16°C.
Thursday 30th August 2007
Friday 31st August 2007
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