Paris/Oligos
From 2007.igem.org
Constructions
- Lox71-FtsZ:
Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
- Lox71-FtsA-FtsZ:
Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
- Deletion of Ecor1 site in FtsZ:
DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
- Lox66-DapAColi:
Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
- Lox66-DapASubtilis:
Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84
DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
- Lox71-KmR-Lox66:
Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
- Deletion of Pst1 site in DGAT:
DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
| Table of Oligos | ||||
|---|---|---|---|---|
| Number | Name | Sequence | Length | Comments |
| 1 | Lox71-FtsZ-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT | 89 | |
| 2 | FtsZ-R | TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT | 49 | |
| 3 | Lox71-FtsA-F | TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG | 89 | |
| 4 | DEcoR1-FtsZ-R | CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC | 40 | |
| 5 | DEcoR1-FtsZ-F | TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA | 40 | |
| 6 | Lox66-DapAColi-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT | 84 | |
| 7 | DapAColi-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC | 52 | |
| 8 | Lox66-DapASubtilis-F | TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA | 84 | |
| 9 | DapASubtilis-R | TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT | 52 | |
| 10 | Lox71-KmR-F | TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC | 85 | |
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| 12 | ||||
| 13 | ||||
