From 2007.igem.org

Transforming BioBricks

1. split the competent E. coli cells (~75ul) in two.
2. competent cells (~33ul) into eppendorfs on ice..
3. add 1ul of plasmid DNA.
4. incubate on ice for at least 5 mins.
5. heat shock +42 ºC for 45 secs.
6. back on ice for 2 mins to reduce damages.
7. add 1ml of soc media.
8. incubate +37ºC in a shaker for 1 hour.
9. spread the reaction on kan/carb LB plates.
10. grow +37 ºC overnight.