| Name
| Sequence
| Tm
|
| *PhZ-Up
| 5' TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
| 65.6
|
| *PhZ-Low
| 5' CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CA G 3'
| 64.8
|
| *Bbp_PhZ_Up
| 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
| 63.6
|
| *Bbs_PhZ_Low
| 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
| 64.2
|
| Bbp_Gene
| 5' gaa ttc gcg gcc gct tct ag 3'
| 65.7
|
| Bbs
| 5' ctg cag cgg ccg cta cta gta 3'
| 65.3
|
| *Methyl_1
| 5' TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
| 66.3
|
| *Methyl_2
| 5' TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
| 66.2
|
| *Bbp_Methyl_1
| 5' gaa ttc gcg gcc gct tct aga TTT TTG AAT TCT TTC GGA CGC AGG AAA AG 3'
| 63.9
|
| *Bbs_Methyl_2
| 5' ctg cag cgg ccg cta cta gta TTT TTG GAT CCG TTG AAA GTT CCG ATT CA 3'
| 66.9
|
| Phz_M_for_1
| 5' atg aat aat tcg aat ctt gct gct 3'
| 65.4
|
| Phz_M_rev_1
| 5' tca ggc cct ggc agc gac 3'
| 60.3
|
| Bbp_Phz_M_for_1
| 5' gaa ttc gcg gcc gct tct aga tga ata att cga atc ttg ctg ct 3'
| 64.5
|
| Bbs_Phz_M_rev_1
| 5' ctg cag cgg ccg cta cta gta tca ggc cct ggc agc gac 3'
| 60.3
|
| *Oxy_1
| 5' TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
| 60.2
|
| *Oxy_2
| 5' TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
| 59.2
|
| *Bbp_Oxy_1
| 5' gaa ttc gcg gcc gct tct aga TCG AAC ACT CTA GAA AAG GAA GCA CC 3'
| 65.1
|
| *Bbs_Oxy_2
| 5' ctg cag cgg ccg cta cta gta TTT TTT GCA TGC TAG CGT GGC CGT TCC 3'
| 66.9
|
| Phz_S_for_1
| 5' atg agc gaa ccc atc gat at 3'
| 71.2
|
| Phz_S_rev_1
| 5' cta gcg tgg ccg ttc cac ctg 3'
| 60.2
|
| Bbp_Phz_S_for_1
| 5' gaa ttc gcg gcc gct tct aga tga gcg aac cca tcg ata t 3'
| 70.9
|
| Bbs_Phz_S_rev_1
| 5' ctg cag cgg ccg cta cta gta cta gcg tgg ccg ttc cac ctg 3'
| 63.3
|
| Phz_H_up
| 5' CGC ACG GAT CCT TTC AGA ATG TTC 3'
| 69.9
|
| Phz_H_low
| 5' GCC ACG CCA AGC TTC ACG CTC A 3'
| 70.6
|
| Bbp_Phz_Up
| 5' gaa ttc gcg gcc gct tct aga TAA GGA TCC GGT AGT TCC AAG CCC CAG AAA C 3'
| 63.3
|
| Bbs_Phz_Low
| 5' ctg cag cgg ccg cta cta gta CAC ATT TGA TCT AGA TGG GTC ACG GCT ATT CAG 3'
| 57.7
|
