Glasgow/Wetlab/Orders
From 2007.igem.org
Emma's primers
| Name | Sequence | Tm |
|---|---|---|
| *(***)-Up | 5' *** C 3' | 65.6 |
| *(***)-Low | 5' *** 3' | 64.8 |
| *Bbp_(***)_Up | 5' *** 3' | 63.6 |
| *Bbs_(***)_Low | 5' *** 3' | 64.2 |
| Bbp_Gene | 5' gaa ttc gcg gcc gct tct ag 3' | 65.7 |
| Bbs | 5' ctg cag cgg ccg cta cta gta 3' | 65.3 |
| *Methyl_1 | 5' *** 3' | 66.3 |
| *Methyl_2 | 5' *** 3' | 66.2 |
| *Bbp_Methyl_1 | 5' *** 3' | 63.9 |
| *Bbs_Methyl_2 | 5' *** 3' | 66.9 |
| (***)_M_for_1 | 5' *** 3' | 65.4 |
| (***)_M_rev_1 | 5' *** 3' | 60.3 |
| Bbp_(***)_M_for_1 | 5' *** 3' | 64.5 |
| Bbs_(***)_M_rev_1 | 5' *** 3' | 60.3 |
| *Oxy_1 | 5' *** 3' | 60.2 |
| *Oxy_2 | 5' *** 3' | 59.2 |
| *Bbp_Oxy_1 | 5' *** 3' | 65.1 |
| *Bbs_Oxy_2 | 5' *** 3' | 66.9 |
| (***)_S_for_1 | 5' *** 3' | 71.2 |
| (***)_S_rev_1 | 5' *** 3' | 60.2 |
| Bbp_(***)_S_for_1 | 5' *** 3' | 70.9 |
| Bbs_(***)_S_rev_1 | 5' *** 3' | 63.3 |
| (***)_H_up | 5' *** 3' | 69.9 |
| (***)_H_low | 5' *** 3' | 70.6 |
| Bbp_(***)_Up | 5' *** 3' | 63.3 |
| Bbs_(***)_Low | 5' *** 3' | 57.7 |
