| Name
| Sequence
| Tm
|
| DmpR_SDM_PstI_1_for
| 5´ CTGGAGCAACAGCATGCGCTGTTC 3´
| 65.6
|
| DmpR_SDM_PstI_1_rev
| 5´ GCTCCAGTTTTCAGCGATGGCC 3´
| 64.8
|
| DmpR_SDM_PstI_2_for
| 5´ CTGGAGGAAGTGCTCAACCAGCAG 3´
| 66.3
|
| DmpR_SDM_PstI_2_rev
| 5´ CCTCCAGCGCTTCCACCAG 3´
| 66.2
|
| DmpR_Start_Prefix_1_for
| 5´ GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG 3´
| 60.2
|
| DmpR_End_Suffix_1_rev
| 5´ CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC 3´
| 59.2
|
| ***M_SDM_PstI_1_for
| 5´ *** 3´
| 69.9
|
| ***M_SDM_PstI_1_rev
| 5´ *** 3´
| 70.6
|
| ***S_SDM_PstI_1_for
| 5´ *** 3´
| 65.7
|
| ***S_SDM_PstI_1_rev
| 5´ *** 3´
| 65.3
|
| ***S_SDM_PstI_2_for
| 5´ *** 3´
| 63.6
|
| ***S_SDM_PstI_2_rev
| 5´ *** 3´
| 63.9
|
| ***C_SDM_PstI_1_for
| 5´ *** 3´
| 65.1
|
| ***C_SDM_PstI_1_rev
| 5´ *** 3´
| 64.2
|
| ***F_SDM_PstI_1_for
| 5´ *** 3´
| 66.9
|
| ***F_SDM_PstI_1_rev
| 5´ *** 3´
| 66.9
|
| ***B_SDM_EcoRI_1_for
| 5´ *** 3´
| 65.4
|
| ***B_SDM_EcoRI_1_rev
| 5´ *** 3´
| 64.5
|
| ***E_SDM_EcoRI_1_for
| 5´ *** 3´
| 60.3
|
| ***E_SDM_EcoRI_1_rev
| 5´ *** 3´
| 60.3
|
| ***E_SDM_PstI_1_for
| 5´ *** 3´
| 71.2
|
| ***E_SDM_PstI_1_rev
| 5´ *** 3´
| 70.9
|
| XylR_SDM_PstI_1_for
| 5´ GAGCTCCAGACCCAGGTTG 3´
| 63.3
|
| XylR_SDM_PstI_1_rev
| 5´ GTCTGGAGCTCGTAGCGCTC 3´
| 63.3
|
| XylR_Prefix_1
| 5´ GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG 3´
| 57.7
|
| XylR_Suffix_1
| 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC 3´
| 59.8
|
| Pu_Prefix_1(After XylR)
| 5´ GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG 3´
| 65.0
|
| Pu_Suffix_1(After XylR)
| 5´ CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG 3´
| 65.1
|
| Pu_Prefix_2(Emma´s sequence)
| 5´ GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC 3´
| 66.0
|
| Pu_Suffix_2(Emma´s sequence)
| 5´ CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC 3´
| 66.4
|
| DntR_Prefix_1
| 5´ GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG 3´
| 61.2
|
| DntR_Suffix_1
| 5´ TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT 3´
| 55.2
|
| DntR_Suffix_2
| 5´ CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG 3´
| 55.3
|
| Pr_Prefix_1
| 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG 3´
| 58.8
|
| Pr_Suffix_1
| 5´ CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG 3´
| 57.3
|
| p1010_for
| 5´ GCTGTGTATAAGGGAGCCTGA 3´
| 59.4
|
| p1010_rev
| 5´ TGACAGTTGACAGCGACAGC 3´
| 61.3
|
| bbp_XylR_for_2
| 5´ GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC 3´
| 55.2
|
| bbs_XylR_rev_2
| 5´ CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG 3´
| 61.8
|
| bbp_Pr_for_2
| 5´ GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG 3´
| 60.8
|
| bbs_Pr_rev_2
| 5´ CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC 3´
| 61.3
|
| bbp_Pu_for_2
| 5´ GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC 3´
| 64.3
|
| bbs_Pu_rev_2
| 5´ CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG 3´
| 69.9
|
| bbp_***D_for
| 5´ *** 3´
| 61.0
|
| bbs_***D_for
| 5´ *** 3´
| 61.4
|
| bbp_***A_for
| 5´ *** 3´
| 63.1
|
| bbs_***G_rev
| 5´ *** 3´
| 61.9
|
