Glasgow/Wetlab/Gels

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Gels

Gels are labelled with numbers for rows and letters for columns.

Date and Reference Description Key Gel
10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 1. 1.(*m*)

2.(*m*)
3.(*s*)
4.(*s*)
5.(*s*)
6.(*s*)
7.neg control
8.neg control
9.neg control

10thAug gel 1.JPG
10th Aug 2007 (2) Colony PCR after ligating (*m*), (*s*), DntR and Pu with the construction vectors 3/20G and 4/6B. Gel 2. 1.(*s*)

2.(*s*)
3.neg control
4.DntR
5.DntR
6.Pu
7.Pu
8.neg control
9.neg control
10.neg control
11.neg control

10thAug gel 2.JPG
13th Aug 2007 (4) PCR of genes (*a→g*), (*a→d*), and (*d→g*). 1A.(*a→g*)

1B.(*a→g*)
1C.(*a→g*)
2A.(*a→g*)
2B.(*a→d*)
2C.(*d→g*)

2007-08-13 12hr 43min.JPG
17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 1. 1.-3.(*m*)+4/6B
(PstI site mutated)

4.-6.(*m*)+3/20G
(PstI site mutated)
7.-9.(*s*)+4/6B
(1st PstI site mutated)
10.-12.(*s*)+3/20G
(2nd PstI site mutated)
13.-15.(*s*)+4/6B
(2nd PstI site mutated)
16.(*s*)+3/20G
(1st PstI site mutated)

17thAug SDM1 PstI digest gel 1.JPG
17th Aug 2007 (2-4) PstI digested minipreps of (*m*) and (*s*) in 4/6B and 3/20G vectors before and after the first round of site-directed mutagenesis. Gel 2. 1.-2.(*s*)+3/20G
(1st PstI site mutated)

3.(*m*)+4/6B
(Before mutagenesis)
4.(*m*)+3/20G
(Before mutagenesis)
5.(*s*)+4/6B
(Before mutagenesis)
6.(*s*)+3/20G
(Before mutagenesis)

17thAug SDM1 PstI digest gel 2.JPG
23rd Aug 2007 (*s*) minipreps in 4/6B and 3/20G run after the second round of site-directed mutagenesis. Maija's Gel taken by Scott.JPG


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