Glasgow/Wetlab/Week9

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Week 9

Monday 27th August 2007

  1. Maija performed a PvuI digest for pDntA+3/20G and pDntA+4/6B constructs with Roche's PvuI enzyme.
Insert Vector Enzyme Expected size Result
pDntA 3/20G PvuI 1450bp + 749bp
pDntA 4/6B PvuI 2375bp + 681bp
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Look at the code to see the template.

Tuesday 28 August 2007

Wednesday 29th August 2007

Scott did colony PCR (see protocol 9) on the assembly transformations carried out yesterday using primers VF2 and VR. This will tell us if the BioBrick insert region is the expected (enlarged) size.

Thursday 30th August 2007

Friday 31st August 2007