Chiba/Week1

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FliC Group AHL Group

Week1(8/16~8/23)

Monday,August,17,2007

  • pUC-FliCのPCR
F/R FliC-207 FliC-228 FliC-240 FliC-252 FliC-267
FliC-207 ①
FliC-228 ② ③
FliC-240 ④ ⑤ ⑥
FliC-252 ⑦ ⑧ ⑨ ⑩
FliC-267 ⑪ ⑫ ⑬ ⑭ ⑮


Recipe(単位：μ) ① ② ③ ④ ⑤ ⑥ ⑦ ⑧ ⑨ ⑩ ⑪ ⑫ ⑬ ⑭ ⑮
pUC19-FliC 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
FliC-207F 5
FliC-207R 5 5 5 5 5
FliC-228F 5 5
FliC-228R 5 5 5 5
FliC-240F 5 5 5
FliC-240R 5 5 5
FliC-252F 5 5 5 5
FliC-252R 5 5
FliC-267F 5 5 5 5 5
FliC-267R 5
10×Buffer 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
dNTPmix 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
Vent 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
dH2O 68 68 68 68 68 68 68 68 68 68 68 68 68 68 68
Total 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
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