Chiba/Week1

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< Chiba
Revision as of 06:19, 16 September 2007 by Maiko (Talk | contribs)
FliC Group AHL Group

8/27(Mon)

  • pUC-FliCのPCR
F/R FliC-207 FliC-228 FliC-240 FliC-252 FliC-267
FliC-207 ①
FliC-228 ② ③
FliC-240 ④ ⑤ ⑥
FliC-252 ⑦ ⑧ ⑨ ⑩
FliC-267 ⑪ ⑫ ⑬ ⑭ ⑮


Recipe(単位：μ) ① ② ③ ④ ⑤ ⑥ ⑦ ⑧ ⑨ ⑩ ⑪ ⑫ ⑬ ⑭ ⑮
pUC19-FliC 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
FliC-207F 5
FliC-207R 5 5 5 5 5
FliC-228F 5 5
FliC-228R 5 5 5 5
FliC-240F 5 5 5
FliC-240R 5 5 5
FliC-252F 5 5 5 5
FliC-252R 5 5
FliC-267F 5 5 5 5 5
FliC-267R 5
10×Buffer 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
dNTPmix 10 10 10 10 10 10 10 10 10 10 10 10 10 10 10
Vent 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
dH2O 68 68 68 68 68 68 68 68 68 68 68 68 68 68 68
Total 100 100 100 100 100 100 100 100 100 100 100 100 100 100 100
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