| Table of Oligos
|
| Number
| Name
| Sequence
| Length
| Comments
|
| O1
| Lox71-FtsZ-F
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT
| 89
|
|
| O2
| FtsZ-R
| TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCA GGAAT
| 49
|
|
| O3
| Lox71-FtsA-F
| TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATT ATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG
| 89
|
|
| O4
| DEcoR1-FtsZ-R
| CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC
| 40
|
|
| O5
| DEcoR1-FtsZ-F
| TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA
| 40
|
|
| O6
| Lox66-DapAColi-F
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAGTTTGCACAGAGGATGGCCCATGTT
| 84
|
|
| O7
| DapAColi-R
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC
| 52
|
|
| O8
| Lox66-DapASubtilis-F
| TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACG AACGGTAATCATGATGAGGTGAATAAAATGAA
| 84
|
|
| O9
| DapASubtilis-R
| TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT
| 52
|
|
| O10
| Lox71-KmR-F
| TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACG AAGTTATCTGATCCTTCAACTCAGCAAAAGTTC
| 85
|
|
| O11
| Lox66-KmR-R
| TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCA AGTTATTTATTAGAAAAACTCATCGAGCATCAAA
| 86
|
|
| O12
| DPst1-DGAT-F
| AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT
| 40
|
|
| O13
| DPst1-DGAT-R
| AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT
| 40
|
|
| O14
| Lox66-F
| AATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAAC GGTATACTAGTAGCGGCCGCTGCA
|
|
|
| O15
| Lox66-R
| GCGGCCGCTACTAGTATACCGTTCGTATAATGTATGCTATACCAAGTTATC TCTAGAAGCGGCCGCG
|
|
|
| O16
| Lox71-F
| AATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAG TTATTACTAGTAGCGGCCGCTGCA
|
|
|
| O17
| Lox71-R
| GCGGCCGCTACTAGTAATAACTTCGTATAATGTATCGTATACGAACGGTAC TCTAGAAGCGGCCGCG
|
|
|
| O18
| VF2
| tgccacctgacgtctaagaa
| 20
| Forward Primer to Biobrick vectors (VF2) BBa_G00100
|
| O19
| VR
| attaccgcctttgagtgagc
| 20
| Reverse Primer to Biobrick Vectors (VR) BBa_G00101
|
| O20
| RBS-DapAColi-F
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGT TCACGGGAAGTATTGTCGCGA
| 71
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Coli) from ATG
|
| O21
| RBS-DapASubtilis-F
| TTTGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAATACTAGATGAA TTTCGGAAATGTGTCTACAG
| 71
| Biobrick prefix + Strong RBS BBa_B0030 + 25 bp homology to DapA (Subtilis) from ATG
|
| O22
| SEQFtsA-1F
| GTCGTCCATACCGCGAAATC
| 20
|
|
| O23
| SEQFtsA-2F
| CGTGGAAGTGCCGAGCGTAG
| 20
|
|
| O24
| SEQFtsZ-1F
| GGTATCGCTGAACTGATTAC
| 20
|
|
| O25
| SEQDapASub-1F
| TTAAAGCAATTGCGGCAGAG
| 20
|
|
| O26
| SEQDapAColi-1F
| CAATCGTCCGTCGCAAGAAG
| 20
|
|
| O27
| ForDGAT1
| ATCCACGCTATGCGCCCATTAC
| 22
| Tm = 60°C
|
| O28
| RevDGAT1
| GGGCTAATTTAGCCCTTTAGTT
| 22
| Tm = 50°C
|
| O29
| ForDGAT_BB1
| AAAGAATTCGCGGCCGCTTCTAGAGATTAAAGAGGAGAAAATCCACGCTATG CGCCCATTAC
| 62
| Tm = 60/83°C
|
| O30
| RevDGAT_BB1
| CTGCAGCGGCCGCTACTAGTAGGGCTAATTTAGCCCTTTAGTT
| 43
| Tm = 50/74°C
|
| O31
| DapAp-F
| aattcgcggccgcttctagagAACAATCAGAACGGTTCTGTCTGCTTGCTTTT AATGCCATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGATGGta
| 104
|
|
| O32
| DapAp-R
| ctagtaCCATCCTCTGTGCAAACAAGTGTCTCAATGGTACGTTTGGTATGGCA TTAAAAGCAAGCAGACAGAACCGTTCTGATTGTTctctagaagcggccgcg
| 104
|
|
| O33
| dDapA-F
| CATACCAAACGTACCATTGAGACACTTGTTTGCACAGAGGAT GGCCCATGATTCCGGGGATCCGTCGACC
| 70
| more here
|
| O34
| dDapA-R
| AAGCCATCAAATCTCCCTAAACTTTACAGCAAACCGGCATGC TTAAGCGCTGTAGGCTGGAGCTGCTTCG
| 70
| more here
|
| O35
| dFtsZ-F
| GACGATGATTACGGCCTCAGGCGACAGGCACAAATCGGAGAGA AACTATGATTCCGGGGATCCGTCGACC
| 70
| more here
|
| O36
| dFtsZ-R
| GAAACCCAAATTCCAGTCAATTCTTAATCAGCTTGCTTACGCA GGAATGCTGTAGGCTGGAGCTGCTTCG
| 70
| more here
|
| O37
| dDapAverif-F
| ACAATCAGAACGGTTCTGTCTG
| 22
|
|
| O38
| dDapAverif-R
| ATAGCGTGAGTCAGAACTACAG
| 22
|
|
| O39
| dFtsZverif-R
| TCGCCAAATTACCTATCCAACC
| 22
|
|
| O40
| SEQ-mRFP-R
| CGGACGACCTTCACCTTCAC
|
|
|
| O41
| M13(-20)-F
| GTAAAACGACGGCCAG
|
|
|
| O42
| M13-R
| CAGGAAACAGCTTATGAC
|
|
|
| O43
| Bfr-FtsK-F
| TTATGGAAGAAGTCAAGCAGAGTAATCGTCTGGTTATTAAGACGCGCTAAT GCCACCTGACGTCTAAGAA
| 70
|
|
| O44
| Bfr-FtsK-R
| CCAGGTTACCCCAGTACTGTTGCTGTATGGATTAACAGGAGTGTAATCAAA TTACCGCCTTTGAGTGAGC
| 70
|
|
| O45
| Aft-FtsK-F
| AGGGGCACAACGGTAATCGTGAAGTGCTGGCCCCACCGCCGTTTGACTAAT GCCACCTGACGTCTAAGAA
| 70
|
|
| O46
| Aft-FtsK-R
| GAAAATACTGAATTTTCAGTTGATAGAGTGCCGGATGCGACGCTACCGCGA TTACCGCCTTTGAGTGAGC
| 70
|
|
| O47
| PacI-pTet-F
| GCTTATTAATTAAGAATTCGCGGCCGCATCTAG
| 33
|
|
| O48
| XmaI-mrfpBB-R
| GGCATCCCGGGCAGCGGCCGCTTCTAGTATA
| 31
|
|
| O49
| Prefix-frt2-R
| TTTCTGGAATTCGCGGCCGCATCTAGAATGGTTCGCTTCAGATCGTT
| 47
|
|
| O50
| SufixBB-frt1-F
| AGGAAGCCTGCAGCGGCCGCTACTAGTA - TCTACAAACTCTGCTAGTTT - GTTTCGAAGTTCCTATACTT
| 48
|
|
| O51
| SufixBB-T1T2frt1-F
| ATTAAGCCTGCAGCGGCCGCTACTAGTAAATGGATCCCCCGGGGGCTG
| 48
|
|
