Paris/October 21

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yesterday -- tomorrow

Contents

Miniprep

The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.

Transformation of Ligation products

Purification of PCR products

Digestion Reactions