From 2007.igem.org
yesterday -- tomorrow
Miniprep
The 80mL culture have been treated as a 10mL culture and lysate have been put through 2 different columns. We eluted the DNA in 2*100µL. We finally recovered 2µg of DNA in 200µL for a concentration of 10ng/µL.
Transformation of Ligation products
Purification of PCR products
Digestion Reactions
| Digestion Products
|
| Number
| Product Name
| Matrix Name
| Enzyme 1
| Enzyme 2
| Size
| Description
|
|
|
|
| D82
| frt1-CmR-frt2 FI
| P16
| EcoRI
| SpeI
|
| frt1-CmR-frt2 PCR product digested for use as a FI
|
|
|
|
| D83
| frt1-CmR-frt2 BI
| P16
| XbaI
| PstI
|
| frt1-CmR-frt2 PCR product digested for use as a BI
|
|
|
|
| D84
| frt1-CmR-frt2 BBi
| P16
| EcoRI
| PstI
|
| frt1-CmR-frt2 PCR product digested for cloning as a BB
|
|
|
|
| D96
| frt1-CmR-frt2-T1T2 FI
| P16
| EcoRI
| SpeI
|
| frt1-CmR-frt2-T1T2 PCR product digested for use as a FI
|
|
|
|
| D97
| frt1-CmR-frt2-T1T2 BI
| P16
| XbaI
| PstI
|
| frt1-CmR-frt2-T1T2 PCR product digested for use as a BI
|
|
|
|
| D98
| frt1-CmR-frt2-T1T2 BBi
| P16
| EcoRI
| PstI
|
| frt1-CmR-frt2-T1T2 PCR product digested for cloning as a BB
|
|
|
|
| D95
| PacI[pY-2C-IntC]SmaI
| pY-2C-IntC plasmid
| PacI
| SmaI
|
| pY-2C-IntC digested for ptet>>lox71-gfpT-lox66-mrfp cloning
|
|
|
|
