From 2007.igem.org
| The official wiki of the NCBS iGEM 2007 Team
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| [http://www.ncbs.res.in/ National Centre for Biological Sciences, Bangalore]
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| The Bangalore iGEM Journal, 07
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e-notebook
| May 07
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| June 07
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| July 07
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Current Status
Tasks Accomplished
Experiments so far
- Calibration of the number of colonies obtained at different optical densities.
- Transformation of competent E.coli (strain K12Z1) cells with constructs.
- Induction of the construct pLac cfp
E.coli cells transformed with the construct pLac cfp were cultured for 10 hours in Luria Bertani medium containing 50mg/ml Ampicillin.
The cells were then transferred into a minimal medium (M9 medium) and were induced with the following concentrations of IPTG. A stock concentration of 100mM was used
| Sample
| 100mM IPTG (ul)
| 1mM IPTG (ul)
| Inoculum volume from LB (ul)
| Volume of M9 added (ml)
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| pLac cfp (0 uM)
| -
| -
| 3 ul
| 3 ml
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| pLac cfp (1 uM)
| -
| -
| 3 ul
| 3 ml
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| pLac cfp (10 uM)
| -
| -
| 3 ul
| 3 ml
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| pLac cfp (50 uM)
| -
| -
| 3 ul
| 3 ml
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| pLac cfp (100 uM)
| -
| -
| 3 ul
| 3 ml
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| pLac cfp (1000 uM)
| -
| -
| 3 ul
| 3 ml
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| pLac cfp (0 uM)
| -
| -
| 3 ul
| 3 ml
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The Week Ahead
The to-do List
Discussions
