Wisconsin/Digestion

From 2007.igem.org

< Wisconsin
Revision as of 18:39, 4 July 2007 by Klapoetke (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Endonuclease Digestion

  • 10uL H2O
  • 5uL DNA
  • 2uL 10x buffer
  • 2uL 1:10 dilution of BSA soln
  • 0.5uL Enzyme 1
  • 0.5uL Enzyme 2

Incubate at 37C for 2-4 hours

Use [http://www.promega.com/guides/re_guide/RESearch.asp this website] for restriction enzyme and [http://www.promega.com/guides/re_guide/RESearch.asp?search=buffer this site] for checking buffer compatibility.

Retrieved from "http://2007.igem.org/wiki/index.php/Wisconsin/Digestion"