Melbourne/primary DNA marker
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< Melbourne
Revision as of 23:41, 10 July 2007 by PhillipDodson (Talk | contribs)
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Contents |
Physical data
- Description:1Kb Plus DNA Ladder, 1ug/ml , 250ml , Store at -20 deg C
- apply 0.1ug of ladder per mm of lane width.
- Recomend BlueJuice loading buffer at 2x.
Method
- Recieved ladder split into twelve 20uL alliquots and stored in ependorfs in Igem Freezer Box 1.
- To one alliquot 400uL of 1x Green Loading dye is added which turns it Blue.
- Place 20uL of this solution into the 10mm well. (1ug/well)(0.1ug/mm of well)
- link to user datasheet/manuals:
- Approximate cost: $0.00 AUD/mg
Lab Location
- Location
- Physical apearance:
- Permision to use required? No
Store
- Available from Bio21 store? YES
- Order number in store:
- Order QTY: 1
Supplier:
- Supplier:
- Suppliers part no:
- Contact details for supplier:
- Email:
- Phone:
Manufacturer:
- Manufacturer: Invitrogen
- Manufacturers Part No: Cat no. 10787-018
- Contact details for Manufacturer: www.invitrogen.com
- [http://www.invitrogen.com/content/sfs/manuals/10787018.pdf| Manufacturers Manual]
- Email:
- Phone:
- PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007