Paris/Oligos

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Constructions

  • Lox71-FtsZ:

Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89

FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49

  • Lox71-FtsA-FtsZ:

Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89

  • Deletion of Ecor1 site in FtsZ:

DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40

DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40


  • Lox66-DapAColi:

Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84

DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52

  • Lox66-DapASubtilis:

Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84

DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52


  • Lox71-KmR-Lox66:

Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85

Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86


  • Deletion of Pst1 site in DGAT:

DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40

DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40

Table of Oligos
Number Name Sequence Length Comments
1 Lox71-FtsZ-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAAACAGGCACAAATCGGAGAGAAACT 89
2 FtsZ-R TTTCTGCAGCGGCCGCTACTAGTATTTTTAATCAGCTTGCTTACGCAGGAAT 49
3 Lox71-FtsA-F TTTGAATTCGCGGCCGCTTCTAGAGTACCGTTCGTATACGATACATTATACGAAGTTATTAATAATCAGGCACAGGCAGAACAACAATG 89
4 DEcoR1-FtsZ-R CGGTATTTACCGCGAAGAACTCAACACCTTCAATGCGCTC 40
5 DEcoR1-FtsZ-F TGCGCGAGCGCATTGAAGGTGTTGAGTTCTTCGCGGTAAA 40
6 Lox66-DapAColi-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAGTTTGCACAGAGGATGGCCCATGTT 84
7 DapAColi-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGCAAACCGGCATGCTTAAGC 52
8 Lox66-DapASubtilis-F TTTGAATTCGCGGCCGCTTCTAGAGATAACTTGGTATAGCATACATTATACGAACGGTAATCATGATGAGGTGAATAAAATGAA 84
9 DapASubtilis-R TTTCTGCAGCGGCCGCTACTAGTATTATTACAGTTCGCTGATCGTGCTGCTT 52
10 Lox71-KmR-F TTTCTCTAGAAGCGGCCGCGAATTCTACCGTTCGTATACGATACATTATACGAAGTTATCTGATCCTTCAACTCAGCAAAAGTTC 85
11 Lox66-KmR-R TTTTACTAGTAGCGGCCGCTGCAGTACCGTTCGTATAATGTATGCTATACCAAGTTATTTATTAGAAAAACTCATCGAGCATCAAA 86
12 DPst1-DGAT-F AGTGCTGTCGTATATGGCCCAGCAGGACTCAACATAATTT 40
13 DPst1-DGAT-R AAATTATGTTGAGTCCTGCTGGGCCATATACGACAGCACT 40
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