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The official wiki of the NCBS iGEM 2007 Team
[http://www.ncbs.res.in/ National Centre for Biological Sciences, Bangalore]
Bangalore The Company The Mission e-Notebook


The Bangalore iGEM Journal, 07




Contents

e-notebook

May 07 June 07 July 07
S M T W T F S S M T W T F S S M T W T F S
1 2 3 4 5 1 2 1 2 3 4 5 6 7
6 7 8 9 10 11 12 3 4 5 6 7 8 9 8 9 10 11 12 13 14
13 14 15 16 17 18 19 10 11 12 13 14 15 16 15 16 17 18 19 20 21
20 21 22 23 24 25 26 17 18 19 20 21 22 23 22 23 24 25 26 27 28
27 28 29 30 31 24 25 26 27 28 29 30 29 30

Current Status

Tasks Accomplished

Experiments so far

  1. Calibration of the number of colonies obtained at different optical densities.
  2. Transformation of competent E.coli (strain K12Z1) cells with constructs.
  3. Induction of the construct pLac cfp

E.coli cells transformed with the construct pLac cfp were cultured for 10 hours in Luria Bertani medium containing 50mg/ml Ampicillin.

The cells were then transferred into a minimal medium (M9 medium) and were induced with the following concentrations of IPTG. A stock concentration of 100mM was used

Sample 100mM IPTG (ul) 1mM IPTG (ul) Inoculum volume from LB (ul) Volume of M9 added (ml)
pLac cfp (0 uM) - - 3 ul 3 ml
pLac cfp (1 uM) - - 3 ul 3 ml
pLac cfp (10 uM) - - 3 ul 3 ml
pLac cfp (50 uM) - - 3 ul 3 ml
pLac cfp (100 uM) - - 3 ul 3 ml
pLac cfp (1000 uM) - - 3 ul 3 ml
pLac cfp (0 uM) - - 3 ul 3 ml

The Week Ahead

The to-do List

Discussions