Glasgow/Wetlab/Orders

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Emma's primers

Name Sequence Tm
*(***)-Up 5' *** C 3' 65.6
*(***)-Low 5' *** 3' 64.8
*Bbp_(***)_Up 5' *** 3' 63.6
*Bbs_(***)_Low 5' *** 3' 64.2
Bbp_Gene 5' gaa ttc gcg gcc gct tct ag 3' 65.7
Bbs 5' ctg cag cgg ccg cta cta gta 3' 65.3
*Methyl_1 5' *** 3' 66.3
*Methyl_2 5' *** 3' 66.2
*Bbp_Methyl_1 5' *** 3' 63.9
*Bbs_Methyl_2 5' *** 3' 66.9
(***)_M_for_1 5' *** 3' 65.4
(***)_M_rev_1 5' *** 3' 60.3
Bbp_(***)_M_for_1 5' *** 3' 64.5
Bbs_(***)_M_rev_1 5' *** 3' 60.3
*Oxy_1 5' *** 3' 60.2
*Oxy_2 5' *** 3' 59.2
*Bbp_Oxy_1 5' *** 3' 65.1
*Bbs_Oxy_2 5' *** 3' 66.9
(***)_S_for_1 5' *** 3' 71.2
(***)_S_rev_1 5' *** 3' 60.2
Bbp_(***)_S_for_1 5' *** 3' 70.9
Bbs_(***)_S_rev_1 5' *** 3' 63.3
(***)_H_up 5' *** 3' 69.9
(***)_H_low 5' *** 3' 70.6
Bbp_(***)_Up 5' *** 3' 63.3
Bbs_(***)_Low 5' *** 3' 57.7

Designed primers

Name Sequence Tm
DmpR_SDM_PstI_1_for 5´CTGGAGCAACAGCATGCGCTGTTC3´ 65.6
DmpR_SDM_PstI_1_rev 5´GCTCCAGTTTTCAGCGATGGCC3´ 64.8
DmpR_SDM_PstI_2_for 5´CTGGAGGAAGTGCTCAACCAGCAG3´ 66.3
DmpR_SDM_PstI_2_rev 5´CCTCCAGCGCTTCCACCAG3´ 66.2
DmpR_Start_Prefix_1_for 5´GAATTCGCGGCCGCTTCTAGATGCCGATCAAGTACAAGCCTG3´ 60.2
DmpR_End_Suffix_1_rev 5´CTGCAGCGGCCGCTACTAGTACTAGCCTTCGATGCCGATTTTC3´ 59.2
***M_SDM_PstI_1_for 5´***3´ 69.9
***M_SDM_PstI_1_rev 5´***3´ 70.6
***S_SDM_PstI_1_for 5´***3´ 65.7
***S_SDM_PstI_1_rev 5´***3´ 65.3
***S_SDM_PstI_2_for 5´***3´ 63.6
***S_SDM_PstI_2_rev 5´***3´ 63.9
***C_SDM_PstI_1_for 5´***3´ 65.1
***C_SDM_PstI_1_rev 5´***3´ 64.2
***F_SDM_PstI_1_for 5´***3´ 66.9
***F_SDM_PstI_1_rev 5´***3´ 66.9
***B_SDM_EcoRI_1_for 5´***3´ 65.4
***B_SDM_EcoRI_1_rev 5´***3´ 64.5
***E_SDM_EcoRI_1_for 5´***3´ 60.3
***E_SDM_EcoRI_1_rev 5´***3´ 60.3
***E_SDM_PstI_1_for 5´***3´ 71.2
***E_SDM_PstI_1_rev 5´***3´ 70.9
XylR_SDM_PstI_1_for 5´GAGCTCCAGACCCAGGTTG3´ 63.3
XylR_SDM_PstI_1_rev 5´GTCTGGAGCTCGTAGCGCTC3´ 63.3
XylR_Prefix_1 5´GAATTCGCGGCCGCTTCTAGATGTCGCTTACATACAAACCCAAG3´ 57.7
XylR_Suffix_1 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCAC3´ 59.8
Pu_Prefix_1(AfterXylR) 5´GAATTCGCGGCCGCTTCTAGAGCGGATTCTGGGGAAAGAG3´ 65.0
Pu_Suffix_1(AfterXylR) 5´CTGCAGCGGCCGCTACTAGTACCTTTACTGCGTGCGAGATCTCGTAGGTG3´ 65.1
Pu_Prefix_2(Emma´s sequence) 5´GAATTCGCGGCCGCTTCTAGAGCCCGGGAAAGCGCGATGAAC3´ 66.0
Pu_Suffix_2(Emma´s sequence) 5´CTGCAGCGGCCGCTACTAGTAGGCGTAACGGCTACGGCTGGC3´ 66.4
DntR_Prefix_1 5´GAATTCGCGGCCGCTTCTAGATGGATCTGCGCGACATCG3´ 61.2
DntR_Suffix_1 5´TACTAGTAGCGGCCGCTGCAGGCTCGAAAAGAGACTTCGTATT3´ 55.2
DntR_Suffix_2 5´CTGCAGCGGCCGCTACTAGTATTATGCTTCAGAGAAAAGCTCG3´ 55.3
Pr_Prefix_1 5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTG3´ 58.8
Pr_Suffix_1 5´CTGCAGCGGCCGCTACTAGTAGTTGTTTTCCTCTTGTTTTTATCGGG3´ 57.3
p1010_for 5´GCTGTGTATAAGGGAGCCTGA3´ 59.4
p1010_rev 5´TGACAGTTGACAGCGACAGC3´ 61.3
bbp_XylR_for_2 5´GAATTCGCGGCCGCTTCTAGATCTCGCTTACATACAAACCC3´ 55.2
bbs_XylR_rev_2 5´CTGCAGCGGCCGCTACTAGTACTATCGGCCCATTGCTTTCACAG3´ 61.8
bbp_Pr_for_2 5´GAATTCGCGGCCGCTTCTAGAGATTTTAATGTGGGCTGCTTGGTGTG3´ 60.8
bbs_Pr_rev_2 5´CTGCAGCGGCCGCTACTAGTAGTAGGAGTACGACGTAGAACCCAAAC3´ 61.3
bbp_Pu_for_2 5´GAATTCGCGGCCGCTTCTAGAGGGAAAGCGCGATGAACCTTTTTTATCGC3´ 64.3
bbs_Pu_rev_2 5´CTGCAGCGGCCGCTACTAGTAGGACTCCAGGCGTAACGGCTACGG3´ 69.9
bbp_***D_for 5´***3´ 61.0
bbs_***D_for 5´***3´ 61.4
bbp_***A_for 5´***3´ 63.1
bbs_***G_rev 5´***3´ 61.9