Melbourne/Making glycerol Stocksl
From 2007.igem.org
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=====Method including controls===== | =====Method including controls===== | ||
| - | #Add to a sterile [[Melbourne/Screw Top tubes|Screw Top tube]] | + | #Add to a sterile [[Melbourne/Screw Top tubes|Screw Top tube]] <BR>*800ul of overnight bacterial culture <BR>*600ul of glycerol |
| - | *800ul of overnight bacterial culture | + | |
| - | *600ul of glycerol | + | |
| - | + | ||
#Mix by inversion | #Mix by inversion | ||
#Immerse in liquid nitrogen and store in -70C freezer. Alternatively, cover the tube with dry ice and pour ethanol in the dry ice. Store immediately in -70C freezer. | #Immerse in liquid nitrogen and store in -70C freezer. Alternatively, cover the tube with dry ice and pour ethanol in the dry ice. Store immediately in -70C freezer. | ||
Revision as of 11:49, 29 September 2007
<Return to list of protocols> <Team home page>
- Applications: Create a stock of recombinant bacteria that can be stored at -70C
- Time to complete protocol: 5min
- Lab time: 5 minutes
- Waiting time: 0
- Approximate cost of materials: $
Contents |
Method from primary and secondary reagents
Primary & secondary Reagents Required including controls
- Overnight bacterial culture
- Glycerol
- Liquid Nitrogen or Dry Ice and Ethanol
Method including controls
- Add to a sterile Screw Top tube
*800ul of overnight bacterial culture
*600ul of glycerol - Mix by inversion
- Immerse in liquid nitrogen and store in -70C freezer. Alternatively, cover the tube with dry ice and pour ethanol in the dry ice. Store immediately in -70C freezer.
Equipement Required
- Screw Top tube
- Pipettes
