Melbourne/primary DNA marker
From 2007.igem.org
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[[Melbourne/Primary Reagents & disposables|<return to list of primary reagents>]] [[Melbourne| <team home page>]] | [[Melbourne/Primary Reagents & disposables|<return to list of primary reagents>]] [[Melbourne| <team home page>]] | ||
====Physical data==== | ====Physical data==== | ||
| - | *Description: | + | *Description:1Kb Plus DNA Ladder, 1ug/ml , 250ml , Store at -20 deg C |
| + | *apply 0.1ug of ladder per mm of lane width. | ||
| + | *Recomend BlueJuice loading buffer at 2x. | ||
| + | [https://catalog.invitrogen.com/productImages/2900/2852.JPG link to ladder jpg] | ||
| + | ====Method==== | ||
| + | *Recieved ladder split into twelve 20uL alliquots and stored in ependorfs in Igem Freezer Box 1. | ||
| + | *To one alliquot 400uL of 1x Green Loading dye is added which turns it Blue. | ||
| + | *Place 20uL of this solution into the 10mm well. (1ug/well)(0.1ug/mm of well) | ||
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*link to user datasheet/manuals: | *link to user datasheet/manuals: | ||
*Approximate cost: $0.00 AUD/mg | *Approximate cost: $0.00 AUD/mg | ||
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====Lab Location==== | ====Lab Location==== | ||
*Location | *Location | ||
| - | + | *Permision to use required? No | |
| - | *Permision to use required? | + | |
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====Store==== | ====Store==== | ||
| - | *Available from Bio21 store? | + | *Available from Bio21 store? YES |
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====Manufacturer:==== | ====Manufacturer:==== | ||
| - | *Manufacturer: | + | *Manufacturer: Invitrogen |
| - | *Manufacturers Part No: | + | *Manufacturers Part No: Cat no. 10787-018 |
| - | *Contact details for Manufacturer: | + | *Contact details for Manufacturer: www.invitrogen.com |
| - | * | + | *[http://www.invitrogen.com/content/sfs/manuals/10787018.pdf Manufacturers Manual] |
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Latest revision as of 23:57, 11 July 2007
<return to list of primary reagents> <team home page>
Contents |
Physical data
- Description:1Kb Plus DNA Ladder, 1ug/ml , 250ml , Store at -20 deg C
- apply 0.1ug of ladder per mm of lane width.
- Recomend BlueJuice loading buffer at 2x.
Method
- Recieved ladder split into twelve 20uL alliquots and stored in ependorfs in Igem Freezer Box 1.
- To one alliquot 400uL of 1x Green Loading dye is added which turns it Blue.
- Place 20uL of this solution into the 10mm well. (1ug/well)(0.1ug/mm of well)
- link to user datasheet/manuals:
- Approximate cost: $0.00 AUD/mg
Lab Location
- Location
- Permision to use required? No
Store
- Available from Bio21 store? YES
Manufacturer:
- Manufacturer: Invitrogen
- Manufacturers Part No: Cat no. 10787-018
- Contact details for Manufacturer: www.invitrogen.com
- Manufacturers Manual
- PhillipDodson 03:48, 1 July 2007 (EDT):Primary Reagent Template 1/July/2007
