USTC

From 2007.igem.org

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<h3>Events</h3>
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'''11/08/2007'''<br>
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'''25/08/2007'''<br>
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Double reporter system:
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The fluorescent reporter (GFP-aav) in double-reporters system does not work. This fact was confirmed by fluorescence assays. New version is under construction.
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The E.Coli cultured from the blue colony shows only very weak green fluorensce intensity. We are considering applying some high copy-number plasmid where the double genes are held.
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'''22/08/2007'''<br>
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Most members of the co-repression promoter family show that they can be repressed by either designed repressor. This result shows that NOR and NOT gates have been got, but no one is suitable for NAND gate. We can considering to modify our scheme to search NAND gate.
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'''01/08/2007'''<br>
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'''11/08/2007'''<br>
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There is a mistake in my preparing X-Gal solution. Please rework your correlative experiments. Sorry! (by Ma Rui)
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Double-reporters system:
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'''30/07/2007'''<br>
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The E.Coli cultured from the blue colony shows only very weak green fluoresce intensity. We are considering applying some high copy-number plasmid where the double genes are held. 
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First designed repressors. Good work, Bo and Xiaofeng!
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[[USTC/Previous|'''Previous''']]
[[USTC/Previous|'''Previous''']]

Revision as of 05:33, 25 August 2007


USTC Logo.png

There aredays left until the Jamboree!


Our Project:
Extensible Logic Circuit in Bacteria

Logic Artificial Biological Circuit (ABC) and logic Integrated Circuit (IC) are both composed by "components" and "wires". Though we have been enjoying the advantages of Ultra Large Scale IC (ULSI), we still cannot implement in real experiments a somewhat large-scaled logic ABC with several levels of logic gates in it.

Our project is to provide a new method for building up a fully extensible logic ABC. For some biological convenience, we are going to produce such biological logic gates as NAND, NOR, NOT which are exactly the basis for modern logic IC. More importantly, several factors influence the scale of ABC and IC, such as, the size and power of the components, the interference between wires, and standardization of intercommunication. Cis-acting components in ABC may present a smaller scale and power request than trans-acting ones, while the other two factors can be improved by novelly-designed and calibrated wires. We are also going to demonstrate an ABC made by these biological logic gates and wires.

Such an ABC may not be large, but the important factors we implement in it will naturally make it fully extensible. IC has existed for 50 years, and now how huge its impact is on our modern lives! Then what about the biological logic circuits in the next 50 years? A new world is waiting...


DSCN1243.png

From left to right
Back row: Zhao Yun, Liu ZQ, Ma XY
Front row: Ma Rui, Su XF, Ding Bo, Zhan Jian


Locations of visitors to this page

Events

25/08/2007
The fluorescent reporter (GFP-aav) in double-reporters system does not work. This fact was confirmed by fluorescence assays. New version is under construction.

22/08/2007
Most members of the co-repression promoter family show that they can be repressed by either designed repressor. This result shows that NOR and NOT gates have been got, but no one is suitable for NAND gate. We can considering to modify our scheme to search NAND gate.

11/08/2007
Double-reporters system:

The E.Coli cultured from the blue colony shows only very weak green fluoresce intensity. We are considering applying some high copy-number plasmid where the double genes are held.

Previous

Tasks


Project Description


PoPS Converters

Biological Logic Gates

Promoter Designing

Lac Repressor Redesigning

Fluorescent Reporters

All Things Linking Together


Members

Graduates:
Zhan Jian
Ding Bo
Ma Rui
Ma Xiaoyu

Undergraduates:
Su Xiaofeng
Liu Ziqing
Zhao Yun

Faculty Advisors:
Prof. HY Liu
Prof. JR Wu
Prof. ZH Hou

Resources

Modeling Utilities

Protocols

Promoter Design

Service Providers

Gallery

Team Photos

Laboratory

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:USTCPhotos USTC Photos]

[http://sg.ustc.edu.cn/mediawiki/index.php/IGEM_USTC_2007:HuangshanPhotos Huangshan Mountain]

Links

[http://partsregistry.org/cgi/partsdb/pgroup.cgi?pgroup=iGEM2007&group=USTC Parts Made by USTC iGEM 2007]

[http://partsregistry.org iGEM Standard Parts' Catalog ]

IGEM2007 Team List

[http://www.ustc.edu.cn Univ. of Sci. and Tech. of China ]