# Cell by Date: Modelling

## Modelling the spoilage of Aerobically Stored Ground Hamburger Meat

A substantial body of work already exists on the topic of spoilage of ground beef by living organisms. The above figure shows the results of a model developed by Koutsoumanis (1) to simulate the spoilage under dynamic temperature conditions of meat by Pseudomonas, - an organism responsible for spoiling refrigerated packaged beef.

An important property was the almost instantaneous response time of the Pseudomonas' growth parameter. To report accurately our system should therefore also share this property.

Koutsoumanis' model, along with Giannuzzi ‘s (2) are both based on the larger class of Gompertz model. A remarkable feature of such models is that through manipulation of the Gompertz Parameters and assuming a Arrhenius type relationship between Pseudomonas' growth parameter and temperature we can infer the Activation energy of the spoilage reaction(3)(See figure below).

Above we can see an arrhenius plot (the log version of the arrhenius equation) of the certain interesting combinations of the parameters in the modifired Gompertz equation such as the Lag Phase Duration (LPD). Doing such a plot allows us to calculate the activation energy of the spoilage reaction.

As given in our specifications the Activation energy for ground meat seem to be around 30kJ/mol. Again to report accurately our system needs to have a similar activation energy.

## Modelling our system: Energy-limited constitutive expression by pTET-mut3BGFP

Energy-dependent model for CBD
Parameter Description
k1 Maximal constitutive transcription by pTET
KE Half-Saturation Coefficient for Energy Hill function
n Positive Cooperativity Coefficient (Hill-coefficient)
δGFP Decay constant of mut3BGFP

here δGFP = 0.0005

In order to use the previous results on how Pseuodmonas spoils aerobically stored ground beef we need to understand how our sytem behaves in similar scenarios to the models developed by Koutsoumanis and Giannuzi.

For isothermal conditions we have been able to develop a simple model of the gene expression in a cell free extract (see chassis characterisation Cell Free Systems) . An important feature of the model was the introduction of a resource dependent term that curbs the synthesis if the system is depleted.

The m-files used in the following simulations can be accessed on our Software page.

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