Imperial/Cell-Free/Contribution
From 2007.igem.org
< Imperial
Cell-Free: Our Contribution
In vitro Expression
- Attempted to make S30 E. coli cell extract and feeding solution
- Successfully used commercial S30 E. coli cell extract and feeding solution from [http://www.promega.com Promega] for expression of GFP
- Successfully used homemade S30 E. coli cell extract with commercial feeding solution for expression of GFP
- Attempting to characterize the temperature range and timespan of expression of our reporter DNA constructs using commercial S30 E. coli cell extract and feeding solution
Vesicles Formation
- Successfully formed empty vesicles, as well as vesicles encapsulating GFP, in Tris-Cl buffer
- Successfully formed vesicles encapsulating GFP in homemade S30 E. coli cell extract
- Attempting to enclose cell extract in vesicles to attain expression of reporter DNA constructs
- Attempting to find suitable pore proteins to prolong vesicle and expression lifespan
Documentation on Registry of Parts
Documented our findings with the following Registry Additions:
[http://partsregistry.org/Chassis/Cell-Free_Systems Cell-Free Systems]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Homemade_E.coli_S30 Homemade E. coli S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_S30 Commercial E. coli S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Commercial_E.coli_T7_S30 Commercial E. coli T7 S30]
- [http://partsregistry.org/Chassis/Cell-Free_Systems/Vesicle Vesicle Encapsulation]
