Imperial/Infector Detector/Implementation

From 2007.igem.org




Infector Detector: Implementation

DNA Construct Implementation
Construct Design
IC07 design ID.png
IC07 design ID-con2.png
Stage 1: Availability

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Found as [http://partsregistry.org/Part:BBa_T9002 BBa_T9002] in Registry.
Imperial ID Design ID1.png
Found as [http://partsregistry.org/Part:BBa_J37032 BBa_J37032] in Registry.
Imperial ID Design ID2.png
Stage 2: Cloning / Amplification

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BBa_T9002 - Lane 18 (1945bp)
BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation

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Not Required Not Required
Stage 4: Purification of Activator Proteins

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Not Required
Purification of LuxR protein (Protocol and Results)
Stage 5: Quality Control

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IC 2007 Plux in vivo.PNG

  • Testing in vivo: Passed (Protocol and Results)
  • Sequencing of Construct: Partially Sequenced
IC 2007 LuxR testing 1.PNG

  • Testing in vitro: Failed (Protocol and Results)
  • Sequencing of Construct: Completed
Stage 6: Final Implementation Status Ready for Testing Aborted.
LuxR protein not functional.



Chassis Construct Implementation
Chassis Design IC07 CFS components.png
Stage 1: Availability

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Possibility of making Homemade S30 Cell Extract. Commercially available S30 Cell Extract purchasable from [http://www.promega.com/search/Default.aspx?query=s30+extract Promega].
Stage 2: Preparation

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Preparation: Done (Protocol) Preparation: Done (Protocol)
Stage 3: Quality Control

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Stage 4: Final Implementation Status Aborted.
Expression capacity of chassis was low.
Ready for Testing



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