Imperial/Wet Lab/Protocols

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Revision as of 21:16, 22 October 2007 by Jec105 (Talk | contribs)


Wet Lab: Protocols

Below is a databank of all the protocols that have been used in our projects.

General ExperimentsTesting Experiments

Protein Purification

  • Purification of LuxR : Protocol and Results
  • Purification of GFPmut3b: Protocol and Results


Fluorometer Testing

  • Optimisation of Fluorometer: Protocol and Results


Calibration Curve

  • Calibration Curve for GFPmut3b: Protocol and Results
  • Degradation Curve for GFPmut3b :Protocol and Results


Chassis Protocols


pT7 Characterisation

  • pT7 in vitro: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/pT7 Results]

Cell by Date
Implementation

  • In Vivo Testing: Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_1.1 Results]
  • Initial In vitro Testing:Protocols and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.1 Results]

Testing/Validation

  • Testing DNA Concentration in vitro:
  • Operating Temperature Range :Protocol 1 and Protocol 2 and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/DNA_concentrations2 Results]
  • Temperature Steps: Protocols and Results

Infecter Detector
Implementation

  • In vivo Testing: Protocol and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/new_pages/in_vivo Results]
  • Initial In Vitro Testing: Protocol and [http://www.openwetware.org/wiki/IGEM:IMPERIAL/2007/Experimental_Design/Phase1/Results_2.2 Results]

Testing/Validation

  • Testing of Purified LuxR :Protocol and Results
  • Characterization in vitro - AHL titration : Protocol and Results