Chiba
From 2007.igem.org
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Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Introduction
Chiba University iGEM07 team's project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae, which is a popular living organism in Japan as a National Treasure. When you come to Japan, you absolutely come across to Marimo in aquariums, TV programs or Mangas :)
Backgrounds
How Marimos are Made
球形の毬藻は，他の崩れた毬藻断片を核として放射状に伸びることによって出来る．よって成長後の球形毬藻は全て一様で核が存在しない．ちなみに最初の無核球形毬藻は，他の泥や砂などを核として成長し崩れた毬藻断片を核としたと考えられている．（イメージ図）
References:
- 阪井與志雄，マリモの科学，北海道大学図書刊行会, 1991 (Yoshio Sakai, Marimo no Kagaku（"The Science of Marimo"))
- 中沢信午，マリモはなぜ丸い その生態と形態，中公新書，1989 (Shingo Nakazawa, Marimo wa naze marui ("Why marimos are spherical"))
Motivations
Why Make a "Marimo Bacteria"?
Making 3D colonies- for next generation molecular biologists
For years microbiologists have been using agar plates to isolate cells from each other. By spreading the diluted cells on a solid surface, we can make "colonies", dome-shaped gathering of genetically identical cells. Although convenient, this is only two-dimentional. What if we can create three dimentional (spherical) colonies with controlled/ defined size? Thus we can eliminate the plating process that everybody hates. Combined with the microfluidics devices, we might be able to pick, isolate, count, or innoculate each of the floating yet independent colonies to conduct routine works in future molecular biology. Crazy thought? That is exactly what our advisors say.
大腸菌は固体上やプレート上でそれぞれバイオフィルムやコロニーを形成するなど，固体上で集合体を生じることは知られている．しかし液体中で細胞自身が集合する例は調べる限りでは見当たらない．我々は大腸菌のみで毬藻のような独立した有限の形の集合体をつくることを目指した．
（イメージ図）
