Chiba/Communication
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===About Quorum Sensing=== | ===About Quorum Sensing=== | ||
| - | [[Image:Chiba_aboutqs.png|frame|'''Fig2.''']] | + | [[Image:Chiba_aboutqs.png|frame|'''Fig2.''' Constructed circuit of Quorum sensing in our system.]] |
Quorum sensing is a cell-to-cell signaling action of bacteria. | Quorum sensing is a cell-to-cell signaling action of bacteria. | ||
They detect the cell density of the same species and coordinate the expression behavior of their cells. | They detect the cell density of the same species and coordinate the expression behavior of their cells. | ||
| - | Species of Gram- | + | Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated. |
| - | + | Detailed gene circuit is discribed in Fig. 2. | |
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Revision as of 21:58, 26 October 2007
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Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Communication Unit
Our Aim
Our aim is to make bacteria stick around a bacteria core.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
Then they stick together. We use Quorum sensing as a signal.
About Quorum Sensing
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Detailed gene circuit is discribed in Fig. 2.
- more about quorum sensing
Experiments: New Parts!!
Making Recievers
New Parts We Made
- ver.1(BBa_T729006)
- ver2.(BBa_I729005)
From Biobrick
- ver.0(BBa_T9002)
Future Work
- ver3
Making Senders
New Parts We Made
- MetK Sender
From Biobrick
- Sender(BBa_S03623)
Future Work
- Light Sensing Sender (Light -> AHL)
futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro
