Chiba/Communication
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[[Image:chiba_logo.png|center]] | [[Image:chiba_logo.png|center]] | ||
__NOTOC__ | __NOTOC__ | ||
| - | {| style="border:0;width:100%;" cellpadding="20px" cellspacing="0" | + | {| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0" |
| align="center" | | | align="center" | | ||
| - | [[Chiba|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1. | + | [[Chiba|Home]]<br> |
| + | <span style="font-size:120%;font-weight:bold;">[[Chiba/Introduction|Introduction]] | [[Chiba/Project_Design|Project Design]] ( [[Chiba/Engeneering_Flagella|1.Affinity Tag]] | [[Chiba/Communication|2.Communication Module]] | [[Chiba/Quorum_Sensing|3.Size Control]] ) | [[Chiba/Making Marimo|Making Marimos]] | [[Chiba/Goal|Our Goal]]</span><br> | ||
| + | [[Chiba/Acknowledgements|Acknowledgements]] | [[Chiba/Team_Members|Team Members]] | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | [[Chiba/Members_Only|メンバ連絡簿]] | ||
|} | |} | ||
| - | ==Communication | + | ==Communication Unit== |
| - | [[Image:Chiba_prj_com.png|frame|''' | + | ===Our Aim=== |
| - | + | [[Image:Chiba_prj_com.png|frame|'''Fig. 13''' Quorum sensing for activating receivers!]] | |
| + | Our aim is to make bacteria stick around a bacteria core. | ||
| + | Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. | ||
| + | Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. | ||
| + | Then they stick together. We use Quorum sensing as a signal. | ||
<br clear="all"> | <br clear="all"> | ||
| - | ==About Quorum Sensing== | + | ===About Quorum Sensing=== |
| - | [[Image:Chiba_aboutqs.png|frame|''' | + | [[Image:Chiba_aboutqs.png|frame|'''Fig. 14''' Constructed circuit of Quorum sensing in our system.]] |
| - | + | Quorum sensing is a cell-to-cell signaling action of bacteria. | |
| - | + | They detect the cell density of the same species and coordinate the expression behavior of their cells. | |
| - | + | Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated. | |
| + | Detailed gene circuit is discribed in Fig. 2. | ||
| + | <br clear="all"> | ||
| + | *more about quorum sensing | ||
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling] | [http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling] | ||
| + | |||
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing] | [http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing] | ||
| - | == | + | ==Experiments: New Parts!!== |
===Recievers=== | ===Recievers=== | ||
| - | * | + | ====New Parts We Made!!==== |
| - | + | *AHL > GFP generator (constitutive aiiA) : [http://partsregistry.org/Part:BBa_I729006 BBa_T729006] | |
| - | + | ||
**[[Image:BBa I729006 circuit.jpg]] | **[[Image:BBa I729006 circuit.jpg]] | ||
| - | * | + | *AHL > GFP & aiiA generator : [http://partsregistry.org/Part:BBa_I729005 BBa_I729005] |
**[[Image:BBa I729005 circuit.jpg]] | **[[Image:BBa I729005 circuit.jpg]] | ||
| - | * | + | *Sensitive AHL > GFP generator : [http://partsregistry.org/Part:BBa_I729004 BBa_I729004] |
| + | **[[Image:Chiba_BBa_I729004.png]] | ||
| + | |||
| + | ====From Biobrick==== | ||
| + | *BBa_T9002 | ||
| + | **[[Image:T9002 circuit.jpg]] | ||
| + | ====Future Work==== | ||
| + | *inverter-aiiA | ||
**[[Image:Rec inv aiia.jpg|357x40px]] | **[[Image:Rec inv aiia.jpg|357x40px]] | ||
| - | === | + | ===Senders=== |
| - | + | ====New Parts We Made==== | |
| - | + | *MetK Sender ( could not deposit to registry; sequencing half completed ) | |
| - | *MetK Sender | + | |
[[Image:MetK circuit.jpg]] | [[Image:MetK circuit.jpg]] | ||
| - | + | ====From Biobrick==== | |
| - | + | *Sender(BBa_S03623) | |
| + | [[Image:BBa s03623.jpg]] | ||
| + | <!-- | ||
==Experiments== | ==Experiments== | ||
===Sender Assay=== | ===Sender Assay=== | ||
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1.Senderは少なくとも5x10^4は必要である。 | 1.Senderは少なくとも5x10^4は必要である。 | ||
2.Senderの株によってLuxIの活性が違う。 | 2.Senderの株によってLuxIの活性が違う。 | ||
| + | --> | ||
Latest revision as of 05:37, 27 October 2007
|
Home |
Communication Unit
Our Aim
Fig. 13 Quorum sensing for activating receivers!
Our aim is to make bacteria stick around a bacteria core.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
Then they stick together. We use Quorum sensing as a signal.
About Quorum Sensing
Fig. 14 Constructed circuit of Quorum sensing in our system.
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Detailed gene circuit is discribed in Fig. 2.
- more about quorum sensing
Experiments: New Parts!!
Recievers
New Parts We Made!!
- AHL > GFP generator (constitutive aiiA) : BBa_T729006
- AHL > GFP & aiiA generator : BBa_I729005
- Sensitive AHL > GFP generator : BBa_I729004
From Biobrick
- BBa_T9002
Future Work
- inverter-aiiA
Senders
New Parts We Made
- MetK Sender ( could not deposit to registry; sequencing half completed )
From Biobrick
- Sender(BBa_S03623)
