Chiba/Communication

From 2007.igem.org

(Difference between revisions)
(Communication Unit)
Line 11: Line 11:
===Our Aim===
===Our Aim===
[[Image:Chiba_prj_com.png|frame|'''Fig1.''' Micro.]]
[[Image:Chiba_prj_com.png|frame|'''Fig1.''' Micro.]]
-
Our aim is that bacterias stick around the bacterias core. We make bacteria sending a signal(sender) and bacteria receiving a signal(receiver). Senders sticking each other in advance send a signal to receivers, which grow stickey hands. In this way, bacterias stick. We use Quorum sensing as a signal.<br>
+
Our aim is to make bacteria stick around a bacteria core.  
-
違う遺伝子回路を持つ2つの細胞(sender&receiver)に分ける.あらかじめお互い結合しているSenderが,周りのreceiverにシグナルを与えてstickey handsを生やす.そして合体.このシグナルにはQuorum sensingを利用する.
+
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
 +
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
 +
Then they stick together. We use Quorum sensing as a signal.
<br clear="all">
<br clear="all">
===About Quorum Sensing===
===About Quorum Sensing===
[[Image:Chiba_aboutqs.png|frame|'''Fig2.''']]
[[Image:Chiba_aboutqs.png|frame|'''Fig2.''']]
-
Quorum Sinsing is cell-to-cell signaling action of bacteria.
+
Quorum sensing is a cell-to-cell signaling action of bacteria.
-
Detect the cell density of same species and coordinating the behavior of cells within a population.
+
They detect the cell density of the same species and coordinate the expression behavior of their cells.
-
Species of Gram-Negativfe signaling information transfer molecule (autoinducer) is acyl homoserine lactone (AHL).When AHL concentration exceed threshold thus the cell population over a certain number, AHL regulated promoter is activated transcription.
+
Species of Gram-Negativfe signaling transfer molecule (autoinducer) is acyl homoserine lactone (AHL). When the AHL concentration exceeded the threshold, the cell population is over a certain number, the AHL induced promoter plux transcription is activated.
Detail geneticaly circuit discribed in Fig2.
Detail geneticaly circuit discribed in Fig2.

Revision as of 21:50, 26 October 2007

Chiba logo.png

Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿


Communication Unit

Our Aim

Fig1. Micro.

Our aim is to make bacteria stick around a bacteria core. Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal. Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags. Then they stick together. We use Quorum sensing as a signal.

About Quorum Sensing

Fig2.

Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negativfe signaling transfer molecule (autoinducer) is acyl homoserine lactone (AHL). When the AHL concentration exceeded the threshold, the cell population is over a certain number, the AHL induced promoter plux transcription is activated.

Detail geneticaly circuit discribed in Fig2.

クオラムセンシングとは,バクテリアが細胞間で行うシグナル伝達のシステムである.自分と同種の菌の生息密度を感知して、それに応じて物質の産生をコントロールする.情報伝達に使用している化合物(オートインデューサー)はグラム陰性菌の場合AHLである.AHLの濃度がある値以上になる,すなわち細胞密度がある値を超えるとAHL依存性プロモータ(LuxP)のスイッチがオンになり,遺伝子が発現される. 詳しい遺伝子回路は図に示す通り.

  • more about quorum sensing

[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]

[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]

Experiments: New Parts!!

Making Recievers

New Parts We Made

  • ver.1(BBa_T729006)
    • BBa I729006 circuit.jpg
  • ver2.(BBa_I729005)
    • BBa I729005 circuit.jpg

From Biobrick

  • ver.0(BBa_T9002)
    • T9002 circuit.jpg

Future Work

  • ver3
    • Rec inv aiia.jpg

Making Senders

New Parts We Made

  • MetK Sender

MetK circuit.jpg

From Biobrick

  • Sender(BBa_S03623)

BBa s03623.jpg

Future Work

  • Light Sensing Sender (Light -> AHL)

Light sensing sender.jpg

futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく,初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro