Chiba/Communication
From 2007.igem.org
(→About Quorum Sensing) |
(→New Parts We Made) |
||
| Line 35: | Line 35: | ||
===Making Recievers=== | ===Making Recievers=== | ||
====New Parts We Made==== | ====New Parts We Made==== | ||
| - | *ver.1(BBa_T729006) | + | *ver.1([http://partsregistry.org/Part:BBa_I729006 BBa_T729006]) |
**[[Image:BBa I729006 circuit.jpg]] | **[[Image:BBa I729006 circuit.jpg]] | ||
| - | *ver2.(BBa_I729005) | + | *ver2.([http://partsregistry.org/Part:BBa_I729005 BBa_I729005]) |
**[[Image:BBa I729005 circuit.jpg]] | **[[Image:BBa I729005 circuit.jpg]] | ||
| + | |||
====From Biobrick==== | ====From Biobrick==== | ||
*ver.0(BBa_T9002) | *ver.0(BBa_T9002) | ||
Revision as of 22:26, 26 October 2007
|
Introduction | Project Design ( 1.Sticky Hands | 2.Communication | 3.Size Control ) | Making Marimos | Our Goal || Team Members | メンバ連絡簿 |
Communication Unit
Our Aim
Our aim is to make bacteria stick around a bacteria core.
Make a sender, a bacteria core that sends a signal, and a receiver, a bacteria that receive a signal.
Our system works like this: Sender core, which is sticking each other in advance, sends a signal to receivers, which grow stickey tags.
Then they stick together. We use Quorum sensing as a signal.
About Quorum Sensing
Quorum sensing is a cell-to-cell signaling action of bacteria. They detect the cell density of the same species and coordinate the expression behavior of their cells. Species of Gram-Negative signaling transfer molecules (so-called autoinducer) is a series of acyl homoserine lactone (AHL). When the AHL concentration exceeds a threshold, that is, the cell population is over a certain number, the promoter plux transcription is activated.
Detailed gene circuit is discribed in Fig. 2.
- more about quorum sensing
[http://partsregistry.org/Featured_Parts:Cell-Cell-Signaling Cell-Cell-Signaling]
[http://www.che.caltech.edu/groups/fha/quorum.html About Quorum sensing]
Experiments: New Parts!!
Making Recievers
New Parts We Made
- ver.1([http://partsregistry.org/Part:BBa_I729006 BBa_T729006])
- ver2.([http://partsregistry.org/Part:BBa_I729005 BBa_I729005])
From Biobrick
- ver.0(BBa_T9002)
Future Work
- ver3
Making Senders
New Parts We Made
- MetK Sender
From Biobrick
- Sender(BBa_S03623)
Future Work
- Light Sensing Sender (Light -> AHL)
futureでLuxIもスイッチできた方が魅力的だと思うんだ。∵ReceiverにSenderを混ぜるのではなく，初めからReceiverとSenderを混ぜた状態で凝集させることができる。ex.)Photoswitch etc... by tashiro
