From 2007.igem.org

Revision as of 03:18, 27 October 2007 (view source) Maiko (Talk | contribs) ← Older edit		Revision as of 03:24, 27 October 2007 (view source) Maiko (Talk | contribs) (→Design) Newer edit →
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	{| border="1"		{| border="1"
-	|	+	| [[Image:Chiba_stickbacteria.png]]
-	Affinity Tag	+	|
		+	'''1.Affinity Tag.'''
		+	Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.
	|-		|-
-	|	+	| [[Image:Chiba_prj_com.png]]
-	Communication Module	+	|
		+	'''2.Communication Module.'''
		+	Make a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.
	|-		|-
-	|	+	| [[Image:Chiba_proj_ahlconc.png]]
-	Size Controler	+	|
		+	'''3.Size Controler.'''
		+	Make an AHL localized region for quorum sensing.
	|}		|}
	これを組み合わせて以下のように毬藻をつくる：		これを組み合わせて以下のように毬藻をつくる：
-
	==Achievements Overview==		==Achievements Overview==

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Revision as of 03:24, 27 October 2007

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal Acknowledgements | Team Members | iGEM Chiba Website | メンバ連絡簿

Chiba's Project

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
(more...)

Design

Our marimo bacteria requres as follows:

	1.Affinity Tag. Make a His-tagged Flagella. We aimed to stick bacteria by displaying histidines (which bonds each other through metal ions) on the flagellar filament.
	2.Communication Module. Make a Bacterial Communication. We make 2 types of cell(sender&receiver) having different gene circuit. Senders sticking each other in advance send a signal to receivers and receivers grow affinity tags.
	3.Size Controler. Make an AHL localized region for quorum sensing.

これを組み合わせて以下のように毬藻をつくる：

Achievements Overview

Making Affinity Tags

1. Tagとなるヒスチジンペプチドが入った鞭毛をつくる．
   • 発現をチェック
   • ディスプレイをチェック

Making Communication Module

1. Making Senders
   • 1.---
   • 2.---
2. Making Receivers
   • 1.---
   • 2.---

Controlling Size

1. Improve Sender
   • 前駆体合成酵素metKを入れてみた．＞結果？
2. Improve Receiver
   • LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
3. Localize AHL
   • aiiAその１の回路（〜〜になるはず）＞結果
   • aiiAその２の回路
   • その３

Making Marimo

Remaining Task(Work)

Our Goal

Future