Chiba/Sandbox/Home2
From 2007.igem.org
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==Design== | ==Design== | ||
Our marimo bacteria requres as follows: | Our marimo bacteria requres as follows: | ||
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| [[Image:Chiba_stickbacteria.png]] | | [[Image:Chiba_stickbacteria.png]] | ||
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これを組み合わせて以下のように毬藻をつくる： | これを組み合わせて以下のように毬藻をつくる： | ||
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==Achievements Overview== | ==Achievements Overview== | ||
Revision as of 03:46, 27 October 2007
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Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal |
Chiba's Project
Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?)
Motivation.
(more...)
Design
Our marimo bacteria requres as follows:
Achievements Overview
Making Affinity Tags
Fig. The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.
- Make the affinity tag by inserting the his-tag into the flagellar fillament.
Sequence confirmed
- Swarm confirmed
- Flagella strained with anti-flagella antibody
- Phenotype confirmed
- Affnity confirmed
Making Communication Module
- Making Senders
- AHL > GFP generator (constitutive aiiA) : BBa_T729006
- AHL > GFP & aiiA generator : BBa_I729005
- Sensitive AHL > GFP generator : BBa_I729004
[edit] Future Work inverter-aiiA
- 1.---
- 2.---
- Making Receivers
- 1.---
- 2.---
Controlling Size
- Improve Sender
- 前駆体合成酵素metKを入れてみた．＞結果？
- Improve Receiver
- LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
- Localize AHL
- aiiAその１の回路（〜〜になるはず）＞結果
- aiiAその２の回路
- その３
