Chiba/Sandbox/Home2

From 2007.igem.org

(Difference between revisions)
Line 12: Line 12:
Motivation.<br>
Motivation.<br>
(more...)
(more...)
-
 
-
===Marimo Bacteria===
 
-
#Affinity Tag]]
 
-
#Communication Module
 
-
#Size Control
 
-
 
-
 
Our marimo bacteria requres as follows:
Our marimo bacteria requres as follows:
Line 41: Line 34:
これを組み合わせて以下のように毬藻をつくる:
これを組み合わせて以下のように毬藻をつくる:
-->
-->
-
===Methods===
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
-
 
こまかすぎか.
こまかすぎか.
-
==Achievements Overview==
+
==Experiments Overview==
===[[Chiba/Engeneering_Flagella|Making Affinity Tags]]===
===[[Chiba/Engeneering_Flagella|Making Affinity Tags]]===
Line 109: Line 49:
<br clear="all">
<br clear="all">
-
===Making Communication Module===
+
===[[Chiba/Communication|Making Communication Module]]===
-
#Making Senders
+
#Making Receivers
#*[[Image:Chiba_check.png]] AHL > GFP generator (constitutive aiiA) : BBa_T729006
#*[[Image:Chiba_check.png]] AHL > GFP generator (constitutive aiiA) : BBa_T729006
#*[[Image:Chiba_check.png]] AHL > GFP & aiiA generator : BBa_I729005
#*[[Image:Chiba_check.png]] AHL > GFP & aiiA generator : BBa_I729005
#*[[Image:Chiba_check.png]] Sensitive AHL > GFP generator : BBa_I729004
#*[[Image:Chiba_check.png]] Sensitive AHL > GFP generator : BBa_I729004
-
[edit]
+
#*[[Image:Chiba_nocheck.png]] inverter-aiiA
-
Future Work
+
#Making Senders
-
inverter-aiiA
+
#*[[Image:Chiba_nocheck.png]] MetK Sender ( could not deposit to registry )
-
 
+
[[Chiba/Communication|more...]]
-
 
+
-
#*1.---
+
-
#*2.---
+
-
#Making Receivers
+
-
#*1.---
+
-
#*2.---
+
-
#*
+
-
#*
+
-
===Controlling Size===
+
===[[Chiba/Quorum_Sensing|Controlling Size]]===
#Improve Sender
#Improve Sender
#*前駆体合成酵素metKを入れてみた.>結果?
#*前駆体合成酵素metKを入れてみた.>結果?

Revision as of 03:57, 27 October 2007

Chiba logo.png

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal
Acknowledgements | Team Members | [http://chem.tf.chiba-u.jp/igem/ iGEM Chiba Website] | メンバ連絡簿

Project Overview

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
(more...)

Our marimo bacteria requres as follows:

こまかすぎか.

Experiments Overview

Making Affinity Tags

Fig. The short peptide with six histidine (“His-Tag”) was inserted into the fliC D3 domain.
  1. Make the affinity tag by inserting the his-tag into the flagellar fillament.
    • Chiba check.png Sequence confirmed
    • Chiba check.png Swarm confirmed
    • Chiba check.png Flagella strained with anti-flagella antibody
    • Chiba check.png Phenotype confirmed
    • Chiba check.png Affnity confirmed

more...

Making Communication Module

  1. Making Receivers
    • Chiba check.png AHL > GFP generator (constitutive aiiA) : BBa_T729006
    • Chiba check.png AHL > GFP & aiiA generator : BBa_I729005
    • Chiba check.png Sensitive AHL > GFP generator : BBa_I729004
    • Chiba nocheck.png inverter-aiiA
  2. Making Senders
    • Chiba nocheck.png MetK Sender ( could not deposit to registry )

more...

Controlling Size

  1. Improve Sender
    • 前駆体合成酵素metKを入れてみた.>結果?
  2. Improve Receiver
    • LuxRの感度が良くなるmutation(論文より)を入れてみた.>結果
  3. Localize AHL
    • aiiAその1の回路(〜〜になるはず)>結果
    • aiiAその2の回路
    • その3

Making Marimo

Remaining Task(Work)

Our Goal

Future