From 2007.igem.org

Introduction | Project Design ( 1.Affinity Tag | 2.Communication Module | 3.Size Control ) | Making Marimos | Our Goal Acknowledgements | Team Members | iGEM Chiba Website | メンバ連絡簿

Chiba's Project

Our iGEM project is to make a Marimo-ish gathering of bacteria. Marimo is a green spherical shaped algae (shown in Fig.1), which is a popular living organism in Japan as a National Treasure.(<because of its shape?) Motivation.
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Design

Our marimo bacteria requres as follows:

Affinity Tag

Communication Module

Size Controler

これを組み合わせて以下のように毬藻をつくる：

Achievements Overview

Making Affinity Tags

1. Tagとなるヒスチジンペプチドが入った鞭毛をつくる．
   • 発現をチェック
   • ディスプレイをチェック

Making Communication Module

1. Making Senders
   • 1.---
   • 2.---
2. Making Receivers
   • 1.---
   • 2.---

Controlling Size

1. Improve Sender
   • 前駆体合成酵素metKを入れてみた．＞結果？
2. Improve Receiver
   • LuxRの感度が良くなるmutation（論文より）を入れてみた．＞結果
3. Localize AHL
   • aiiAその１の回路（〜〜になるはず）＞結果
   • aiiAその２の回路
   • その３

Making Marimo

Remaining Task(Work)

Our Goal

Future