Imperial/Cell-Free/Applications

From 2007.igem.org

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== Our Design ==
== Our Design ==
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To get around the problem of having bacteria in contact with food or medical devices in the applications of '''Infector Detector''' and '''Cell by Date''' respectively, we aimed to express reporter DNA constructs within a CFS consisting of cell extract encapsulated in selectively permeable vesicles. Our plan was to first prepare the cell extract and perform '''''in vitro'' expression''' in bulk solution. In parallel, we would investigate and optimize conditions for '''phospholipid vesicle formation'''. The final stage of our project would involve combining the two, so that protein expression is achieved inside the vesicles.
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To get around the problem of having bacteria in contact with food or medical devices in the applications of '''Infector Detector''' and '''Cell by Date''' respectively, we aimed to express reporter DNA constructs within a CFS consisting of ''E. coli'' cell extract in bulk solution. In parallel, we would investigate and optimize conditions for '''phospholipid vesicle formation'''. Future work on our project would then involve combining the two, so that protein expression is achieved inside the vesicles for prolonged chassis lifespan.
== Cell-free in Infector Detector ==
== Cell-free in Infector Detector ==

Revision as of 22:08, 20 October 2007


Our Design

To get around the problem of having bacteria in contact with food or medical devices in the applications of Infector Detector and Cell by Date respectively, we aimed to express reporter DNA constructs within a CFS consisting of E. coli cell extract in bulk solution. In parallel, we would investigate and optimize conditions for phospholipid vesicle formation. Future work on our project would then involve combining the two, so that protein expression is achieved inside the vesicles for prolonged chassis lifespan.

Cell-free in Infector Detector

(Specs/Design based)



Cell-free in Cell by Date

(Specs/Design based)





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