Imperial/Cell by Date/Implementation

From 2007.igem.org

(Difference between revisions)
Line 19: Line 19:
__NOTOC__
__NOTOC__
= Cell by Date: Implementation =
= Cell by Date: Implementation =
 +
 +
 +
{|border=1 width=100% align="center" cellpadding="10px"
 +
|-
 +
|colspan="3" style="background-color:#eeeeff"| <center>'''DNA Construct Implementation'''</center>
 +
|- align="center"
 +
|wifth="20%" style="background-color:#ddeeff"| '''Construct Design'''
 +
|width="40%"| [[Image:Imperial CBD Design CBD1.png]]
 +
|width="40%"| [[Image:Imperial CBD Design CBD2.png]]
 +
|- align="center"
 +
|width="20%" style="background-color:#ddeeff"| '''Stage 1: Availability'''
 +
|width="40%"| Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry
 +
|width="40%"| Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
 +
|- align="center"
 +
|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification'''
 +
|width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]]
 +
|width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + 2079bp vector)]]
 +
|- align="center"
 +
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation'''
 +
|width="40%"| Not Required
 +
|width="40%"| Not Required
 +
|- align="center"
 +
|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins'''
 +
|width="40%"| Not Required
 +
|width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results])
 +
|-
 +
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control'''
 +
|width="40%"|
 +
* '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results])
 +
* '''Sequencing of Construct''': Pending
 +
|width="40%"|
 +
* '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])
 +
* '''Sequencing of Construct''': Pending
 +
|- align="center"
 +
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status'''
 +
|width="40%" style="background-color:#b8efb0"| Ready for Testing
 +
|width="40%" style="background-color:#ddb99e"| Aborted. <br /> LuxR protein not functional.
 +
|}
 +
<br clear="all">
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
 +
== DNA Construct Status ==
== DNA Construct Status ==

Revision as of 12:34, 24 October 2007



Cell by Date: Implementation

DNA Construct Implementation
Construct Design Imperial CBD Design CBD1.png Imperial CBD Design CBD2.png
Stage 1: Availability Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry.
Stage 2: Cloning / Amplification
BBa_T9002 - Lane 18 (1945bp)
BBa_J37032 - Lane R25 (938bp + 2079bp vector)
Stage 3: Ligation Not Required Not Required
Stage 4: Purification of Activator Proteins Not Required LuxR protein (Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results])
Stage 5: Quality Control
  • Testing in vivo: Done (Protocol and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results])
  • Sequencing of Construct: Pending
  • Testing in vitro: Done (Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results])
  • Sequencing of Construct: Pending
Stage 6: Final Implementation Status Ready for Testing Aborted.
LuxR protein not functional.









DNA Construct Status

Imperial CBD Design CBD1.png
Notes: This part can be found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in the registry
Imperial CBD Design CBD2.png
Notes: The part [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] we found in the registry was not functional, therefore, we made our own T7 promoter and ligated it to the GFP expression biobrick [http://partsregistry.org/Part:BBa_I13504 BBa_I13504]


Constitutive tetR Promoter

Imperial CBD Design CBD1.png

Ligation Strategy:

N/A

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at the testing page.
 Sequencing Pending Sequence results not obtained yet

Constitutive T7 Promoter

Imperial CBD Design CBD2.png

Ligation Strategy:

Imperial CBD Design CBD4.png + Imperial CBD Design CBD3.png = Imperial CBD Design CBD2.png

Quality Control:

 Testing in vivo Done Results can be found at -----
 Testing in vitro Done Results can be found at -----
 Sequencing Pending Sequence results not obtained yet


Chassis Status

cell extract preparation protocols.

Navigation

<< Modelling | Implementation | Testing/Validation >>