Imperial/Cell by Date/Implementation
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Revision as of 12:34, 24 October 2007 by Alexander.wong (Talk | contribs)
Cell by Date: Implementation
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Construct Design | ||
Stage 1: Availability | Found as [http://partsregistry.org/Part:BBa_I13522 BBa_I13522] in Registry | Found as [http://partsregistry.org/Part:BBa_E7104 BBa_E7104] in Registry. |
Stage 2: Cloning / Amplification | ||
Stage 3: Ligation | Not Required | Not Required |
Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]) |
Stage 5: Quality Control |
|
|
Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
DNA Construct Status
Constitutive tetR Promoter
Ligation Strategy:
N/A
Quality Control:
Testing in vivo | Done | Results can be found at ----- |
Testing in vitro | Done | Results can be found at the testing page. |
Sequencing | Pending | Sequence results not obtained yet |
Constitutive T7 Promoter
Ligation Strategy:
+ | = |
Quality Control:
Testing in vivo | Done | Results can be found at ----- |
Testing in vitro | Done | Results can be found at ----- |
Sequencing | Pending | Sequence results not obtained yet |
Chassis Status
cell extract preparation protocols.