Imperial/Infector Detector/Implementation
From 2007.igem.org
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|width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' | |width="20%" style="background-color:#ddeeff"| '''Stage 2: Cloning / Amplification''' | ||
| - | |width="40%"| | + | |width="40%"| [[Image:IC07_ID1-cloning.png|thumb|center|350px| BBa_T9002 - Lane 18 (1945bp)]] |
| - | |width="40%"| | + | |width="40%"| [[Image:IC07_ID2-cloning.png|thumb|center|290px| BBa_J37032 - Lane R25 (938bp + vector)]] |
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' | |width="20%" style="background-color:#ddeeff"| '''Stage 3: Ligation''' | ||
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|width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' | |width="20%" style="background-color:#ddeeff"| '''Stage 4: Purification of Activator Proteins''' | ||
|width="40%"| Not Required | |width="40%"| Not Required | ||
| - | |width="40%"| LuxR protein (Protocols | + | |width="40%"| LuxR protein ([[Imperial/Wet_Lab/Protocols/Prot1.5|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.5 Results]) |
|- | |- | ||
|width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | |width="20%" style="background-color:#ddeeff" align="center" | '''Stage 5: Quality Control''' | ||
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in vivo''''': Done (Protocols | + | * '''Testing ''in vivo''''': Done ([[Imperial/Wet_Lab/Protocols/ID1.1|Protocol]] and [http://openwetware.org/index.php?title=IGEM:Imperial/2007/Wet_Lab/results/ID1.1 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|width="40%"| | |width="40%"| | ||
| - | * '''Testing ''in | + | * '''Testing ''in vitro''''': Done ([[Imperial/Wet_Lab/Protocols/Prot1.7|Protocol]] and [http://openwetware.org/wiki/IGEM:Imperial/2007/Wet_Lab/results/Res1.7 Results]) |
* '''Sequencing of Construct''': Pending | * '''Sequencing of Construct''': Pending | ||
|- align="center" | |- align="center" | ||
|width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | |width="20%" style="background-color:#ddeeff"| '''Stage 6: Final Implementation Status''' | ||
| - | |width="40%"| Ready for Testing | + | |width="40%" style="background-color:#b8efb0"| Ready for Testing |
| - | |width="40%"| Aborted. <br /> LuxR protein not functional. | + | |width="40%" style="background-color:#ddb99e"| Aborted. <br /> LuxR protein not functional. |
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Revision as of 11:46, 24 October 2007
Infector Detector: Implementation
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| Construct Design | 
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| Stage 1: Availability | Found as BBa_T9002 in Registry. | Found as BBa_J37032 in Registry. |
| Stage 2: Cloning / Amplification |  |  |
| Stage 3: Ligation | Not Required | Not Required |
| Stage 4: Purification of Activator Proteins | Not Required | LuxR protein (Protocol and Results) |
| Stage 5: Quality Control | ||
| Stage 6: Final Implementation Status | Ready for Testing | Aborted. LuxR protein not functional. |
Chassis Status
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(Link to Commercial Preparation Protocols)
